Important role of β 1-integrin in fucoidan-induced apoptosis via caspase-8 activation

Yumi Yamasaki, Masao Yamasaki, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Fucoidan induces apoptosis by activating caspase-8 in human MCF-7 breast cancer cells, but the detailed mechanism for this is not understood. We demonstrate here that fucoidan interacted with the cell surface, and silencing the β 1-integrin gene expression inhibited fucoidan-induced apoptosis accompanied by caspase-8 activation. Fucoidan induced formation of the β 1-integrin-caspase-8 complex. These data indicate that β 1-integrin is an important factor for the cell-surface binding of fucoidan and plays an important role in fucoidan-induced apoptosis. Fucoidan also induced recruitment of caspase-8 to the β 1-integrin intracellular domain, cleaved it into the activated protein by direct combination with β 1-integrin, and induced apoptosis via the caspase cascade in MCF-7 cells.

Original languageEnglish
Pages (from-to)1163-1168
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number6
DOIs
Publication statusPublished - Jun 29 2012

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Caspase 8
Integrins
Chemical activation
Apoptosis
MCF-7 Cells
Caspases
fucoidan
Gene expression
Cells
Breast Neoplasms
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Important role of β 1-integrin in fucoidan-induced apoptosis via caspase-8 activation. / Yamasaki, Yumi; Yamasaki, Masao; Tachibana, Hirofumi; Yamada, Koji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 6, 29.06.2012, p. 1163-1168.

Research output: Contribution to journalArticle

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