Abstract
Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques.
| Original language | English |
|---|---|
| Pages (from-to) | 58-64 |
| Number of pages | 7 |
| Journal | Microbes and Infection |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2011 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Infectious Diseases
Cite this
Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications. / Saito, Akatsuki; Nomaguchi, Masako; Iijima, Sayuki; Kuroishi, Ayumu; Yoshida, Tomoyuki; Lee, Young Jung; Hayakawa, Toshiyuki; Kono, Ken; Nakayama, Emi E.; Shioda, Tatsuo; Yasutomi, Yasuhiro; Adachi, Akio; Matano, Tetsuro; Akari, Hirofumi.
In: Microbes and Infection, Vol. 13, No. 1, 01.01.2011, p. 58-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications
AU - Saito, Akatsuki
AU - Nomaguchi, Masako
AU - Iijima, Sayuki
AU - Kuroishi, Ayumu
AU - Yoshida, Tomoyuki
AU - Lee, Young Jung
AU - Hayakawa, Toshiyuki
AU - Kono, Ken
AU - Nakayama, Emi E.
AU - Shioda, Tatsuo
AU - Yasutomi, Yasuhiro
AU - Adachi, Akio
AU - Matano, Tetsuro
AU - Akari, Hirofumi
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques.
AB - Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques.
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UR - http://www.scopus.com/inward/citedby.url?scp=78650614347&partnerID=8YFLogxK
U2 - 10.1016/j.micinf.2010.10.001
DO - 10.1016/j.micinf.2010.10.001
M3 - Article
C2 - 20955815
AN - SCOPUS:78650614347
VL - 13
SP - 58
EP - 64
JO - Microbes and Infection
JF - Microbes and Infection
SN - 1286-4579
IS - 1
ER -