TY - JOUR
T1 - Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications
AU - Saito, Akatsuki
AU - Nomaguchi, Masako
AU - Iijima, Sayuki
AU - Kuroishi, Ayumu
AU - Yoshida, Tomoyuki
AU - Lee, Young Jung
AU - Hayakawa, Toshiyuki
AU - Kono, Ken
AU - Nakayama, Emi E.
AU - Shioda, Tatsuo
AU - Yasutomi, Yasuhiro
AU - Adachi, Akio
AU - Matano, Tetsuro
AU - Akari, Hirofumi
N1 - Funding Information:
The authors wish to thank T. Kurosawa, M. Fujita and M. Yasue for their helpful assistance. The authors also thank F. Ono, Y. Katakai, K. Komatsuzaki, A. Hiyaoka, K. Ohto, H. Ohto, and Y. Emoto for their support in animal experiments. We also thank M. Kaizu for his technical support. The anti-CD8 antibody used in the present study was provided by the NIH Nonhuman Primate Reagent Resource (R24 RR016001, N01 AI040101). This work was supported by grants from the Japan Health Sciences Foundation and the Ministry of Health, Labor, and Welfare in Japan and by Global COE Program A06 of Kyoto University.
PY - 2011/1
Y1 - 2011/1
N2 - Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques.
AB - Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques.
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U2 - 10.1016/j.micinf.2010.10.001
DO - 10.1016/j.micinf.2010.10.001
M3 - Article
C2 - 20955815
AN - SCOPUS:78650614347
VL - 13
SP - 58
EP - 64
JO - Microbes and Infection
JF - Microbes and Infection
SN - 1286-4579
IS - 1
ER -
