Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae

Hiroshi Koyama, Eriko Sumiya, Takahiro Ito, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The PCR-based gene disruption strategy originally devised by Baudin et al. is widely used for gene targeting in Saccharomyces cerevisiae. An advantage of this strategy is its simplicity in making targeting constructs. The efficiencies of the targeted disruption are highly variable from locus to locus, however, and often very low. In this report, a method for improving the gene deletion efficiency is described.

Original languageEnglish
Pages (from-to)193-194
Number of pages2
JournalFEMS Yeast Research
Volume8
Issue number2
DOIs
Publication statusPublished - Mar 1 2008
Externally publishedYes

Fingerprint

Gene Targeting
Gene Deletion
Saccharomyces cerevisiae
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae. / Koyama, Hiroshi; Sumiya, Eriko; Ito, Takahiro; Sekimizu, Kazuhisa.

In: FEMS Yeast Research, Vol. 8, No. 2, 01.03.2008, p. 193-194.

Research output: Contribution to journalArticle

Koyama, Hiroshi ; Sumiya, Eriko ; Ito, Takahiro ; Sekimizu, Kazuhisa. / Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae. In: FEMS Yeast Research. 2008 ; Vol. 8, No. 2. pp. 193-194.
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