Improved serum-free defined medium for proliferation and differentiation of chick primary myogenic cells

Masataka Shiozuka, Ichiro Kimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The development of serum-free and chemically defined media remains an important mission in the in vitro research of cells. The present study describes an improved serum-free medium that supports the proliferation and differentiation of chick embryonic primary skeletal myogenic cells, a cell type which has long been utilized for the study of cell differentiation. We show that serum can be replaced with transferrin, insulin, serum albumin, and fibroblast growth factor-2 as supplements to Dulbecco's modified Eagle minimum essential medium, with no loss in the ability of the medium to support proliferation and differentiation of myogenic cells. This medium has several additional advantages over serum-supplemented medium in that it also suppresses the proliferation of contaminating fibroblasts, and may allow the sensitive evaluation of the effects of various humoral factors on myogenic cells. We believe this medium will prove useful to the primary culture of chick myogenic cells.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalZoological science
Volume17
Issue number2
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Fingerprint

chicks
cells
fibroblast growth factor 2
eagles
transferrin
serum albumin
cell differentiation
fibroblasts
insulin

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Improved serum-free defined medium for proliferation and differentiation of chick primary myogenic cells. / Shiozuka, Masataka; Kimura, Ichiro.

In: Zoological science, Vol. 17, No. 2, 01.01.2000, p. 201-207.

Research output: Contribution to journalArticle

@article{a602aa365d484e008597f00061513191,
title = "Improved serum-free defined medium for proliferation and differentiation of chick primary myogenic cells",
abstract = "The development of serum-free and chemically defined media remains an important mission in the in vitro research of cells. The present study describes an improved serum-free medium that supports the proliferation and differentiation of chick embryonic primary skeletal myogenic cells, a cell type which has long been utilized for the study of cell differentiation. We show that serum can be replaced with transferrin, insulin, serum albumin, and fibroblast growth factor-2 as supplements to Dulbecco's modified Eagle minimum essential medium, with no loss in the ability of the medium to support proliferation and differentiation of myogenic cells. This medium has several additional advantages over serum-supplemented medium in that it also suppresses the proliferation of contaminating fibroblasts, and may allow the sensitive evaluation of the effects of various humoral factors on myogenic cells. We believe this medium will prove useful to the primary culture of chick myogenic cells.",
author = "Masataka Shiozuka and Ichiro Kimura",
year = "2000",
month = "1",
day = "1",
doi = "10.2108/zsj.17.201",
language = "English",
volume = "17",
pages = "201--207",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "2",

}

TY - JOUR

T1 - Improved serum-free defined medium for proliferation and differentiation of chick primary myogenic cells

AU - Shiozuka, Masataka

AU - Kimura, Ichiro

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The development of serum-free and chemically defined media remains an important mission in the in vitro research of cells. The present study describes an improved serum-free medium that supports the proliferation and differentiation of chick embryonic primary skeletal myogenic cells, a cell type which has long been utilized for the study of cell differentiation. We show that serum can be replaced with transferrin, insulin, serum albumin, and fibroblast growth factor-2 as supplements to Dulbecco's modified Eagle minimum essential medium, with no loss in the ability of the medium to support proliferation and differentiation of myogenic cells. This medium has several additional advantages over serum-supplemented medium in that it also suppresses the proliferation of contaminating fibroblasts, and may allow the sensitive evaluation of the effects of various humoral factors on myogenic cells. We believe this medium will prove useful to the primary culture of chick myogenic cells.

AB - The development of serum-free and chemically defined media remains an important mission in the in vitro research of cells. The present study describes an improved serum-free medium that supports the proliferation and differentiation of chick embryonic primary skeletal myogenic cells, a cell type which has long been utilized for the study of cell differentiation. We show that serum can be replaced with transferrin, insulin, serum albumin, and fibroblast growth factor-2 as supplements to Dulbecco's modified Eagle minimum essential medium, with no loss in the ability of the medium to support proliferation and differentiation of myogenic cells. This medium has several additional advantages over serum-supplemented medium in that it also suppresses the proliferation of contaminating fibroblasts, and may allow the sensitive evaluation of the effects of various humoral factors on myogenic cells. We believe this medium will prove useful to the primary culture of chick myogenic cells.

UR - http://www.scopus.com/inward/record.url?scp=0034341679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034341679&partnerID=8YFLogxK

U2 - 10.2108/zsj.17.201

DO - 10.2108/zsj.17.201

M3 - Article

VL - 17

SP - 201

EP - 207

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 2

ER -