Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping

Kentaro Inokuma, James C. Liao, Masahiro Okamoto, Taizo Hanai

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108 Citations (Scopus)

Abstract

To improve isopropanol production by metabolically engineered Escherichia coli strain TA76, the optimization of fermentation conditions and isopropanol removal by gas stripping were performed. Isopropanol is one of the simplest secondary alcohols, and it can be dehydrated to yield propylene, which is currently derived from petroleum as a monomer for making polypropylene. Initially, using a pH-controlled fed-batch culture with the intermittent addition of glucose, strain TA76 produced 667. mM (40.1. g/L) of isopropanol after 60. h, representing 73.2% (mol isopropanol/mol glucose) of the theoretical maximum yield. Because the accumulation of isopropanol drastically reduced production yields, a gas stripping recovery method was incorporated into the fed-batch culture system. Using this approach, strain TA76 produced 2378. mM (143. g/L) of isopropanol after 240. h with a yield of 67.4% (mol/mol). To our knowledge, this titer represents the highest level of isopropanol production by E. coli to date and suggests that strain TA76 has a great potential for commercial fermentative isopropanol production.

Original languageEnglish
Pages (from-to)696-701
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume110
Issue number6
DOIs
Publication statusPublished - Dec 1 2010

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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