Abstract
To improve isopropanol production by metabolically engineered Escherichia coli strain TA76, the optimization of fermentation conditions and isopropanol removal by gas stripping were performed. Isopropanol is one of the simplest secondary alcohols, and it can be dehydrated to yield propylene, which is currently derived from petroleum as a monomer for making polypropylene. Initially, using a pH-controlled fed-batch culture with the intermittent addition of glucose, strain TA76 produced 667. mM (40.1. g/L) of isopropanol after 60. h, representing 73.2% (mol isopropanol/mol glucose) of the theoretical maximum yield. Because the accumulation of isopropanol drastically reduced production yields, a gas stripping recovery method was incorporated into the fed-batch culture system. Using this approach, strain TA76 produced 2378. mM (143. g/L) of isopropanol after 240. h with a yield of 67.4% (mol/mol). To our knowledge, this titer represents the highest level of isopropanol production by E. coli to date and suggests that strain TA76 has a great potential for commercial fermentative isopropanol production.
| Original language | English |
|---|---|
| Pages (from-to) | 696-701 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 110 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 2010 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping. / Inokuma, Kentaro; Liao, James C.; Okamoto, Masahiro; Hanai, Taizo.
In: Journal of Bioscience and Bioengineering, Vol. 110, No. 6, 01.12.2010, p. 696-701.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping
AU - Inokuma, Kentaro
AU - Liao, James C.
AU - Okamoto, Masahiro
AU - Hanai, Taizo
PY - 2010/12/1
Y1 - 2010/12/1
N2 - To improve isopropanol production by metabolically engineered Escherichia coli strain TA76, the optimization of fermentation conditions and isopropanol removal by gas stripping were performed. Isopropanol is one of the simplest secondary alcohols, and it can be dehydrated to yield propylene, which is currently derived from petroleum as a monomer for making polypropylene. Initially, using a pH-controlled fed-batch culture with the intermittent addition of glucose, strain TA76 produced 667. mM (40.1. g/L) of isopropanol after 60. h, representing 73.2% (mol isopropanol/mol glucose) of the theoretical maximum yield. Because the accumulation of isopropanol drastically reduced production yields, a gas stripping recovery method was incorporated into the fed-batch culture system. Using this approach, strain TA76 produced 2378. mM (143. g/L) of isopropanol after 240. h with a yield of 67.4% (mol/mol). To our knowledge, this titer represents the highest level of isopropanol production by E. coli to date and suggests that strain TA76 has a great potential for commercial fermentative isopropanol production.
AB - To improve isopropanol production by metabolically engineered Escherichia coli strain TA76, the optimization of fermentation conditions and isopropanol removal by gas stripping were performed. Isopropanol is one of the simplest secondary alcohols, and it can be dehydrated to yield propylene, which is currently derived from petroleum as a monomer for making polypropylene. Initially, using a pH-controlled fed-batch culture with the intermittent addition of glucose, strain TA76 produced 667. mM (40.1. g/L) of isopropanol after 60. h, representing 73.2% (mol isopropanol/mol glucose) of the theoretical maximum yield. Because the accumulation of isopropanol drastically reduced production yields, a gas stripping recovery method was incorporated into the fed-batch culture system. Using this approach, strain TA76 produced 2378. mM (143. g/L) of isopropanol after 240. h with a yield of 67.4% (mol/mol). To our knowledge, this titer represents the highest level of isopropanol production by E. coli to date and suggests that strain TA76 has a great potential for commercial fermentative isopropanol production.
UR - http://www.scopus.com/inward/record.url?scp=78449244865&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78449244865&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2010.07.010
DO - 10.1016/j.jbiosc.2010.07.010
M3 - Article
VL - 110
SP - 696
EP - 701
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 6
ER -