Improvement of pseudojujubogenin glycosides production from regenerated Bacopa monnieri (L.) Wettst. and enhanced yield by elicitors

Sirilak Kamonwannasit, Watoo Phrompittayarat, Kornkanok Ingkaninan, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Bacopa monnieri (L.) Wettst. was studied for shoot induction and regeneration on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Stem explants cultured on medium containing 0.1 mg/l thidiazuron (TDZ) resulted in the highest number of shoots (117 shoots/explant). Regenerated plants from medium with 0.5 mg/l TDZ contained the highest level of pseudojujubogenin glycosides [(30.62 ± 1.29) mg/g dry wt] which was 2-fold higher than that of in vitro grown plants of the same age [(16.96 ± 1.49) mg/g dry wt]. Plantlets regenerated from 0.1 mg/l TDZ also showed a high level of pseudojujubogenin glycosides [(27.94 ± 1.19) mg/g dry wt]. The effect of elicitor on pseudojujubogenin glycosides accumulation in B. monnieri whole plant cultures was investigated. Chitosan at 150 mg/l and yeast extract at 2 mg/ml increased the pseudojujubogenin glycosides production [(40.83 ± 2.24) mg/g dry wt and (40.05 ± 2.37) mg/g dry wt, respectively] after 7 days, which was 6-fold higher than in the control cultures.

Original languageEnglish
Pages (from-to)879-883
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume63
Issue number11-12
DOIs
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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