Improvement of pseudojujubogenin glycosides production from regenerated Bacopa monnieri (L.) Wettst. and enhanced yield by elicitors

Sirilak Kamonwannasit, Watoo Phrompittayarat, Kornkanok Ingkaninan, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Bacopa monnieri (L.) Wettst. was studied for shoot induction and regeneration on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Stem explants cultured on medium containing 0.1 mg/l thidiazuron (TDZ) resulted in the highest number of shoots (117 shoots/explant). Regenerated plants from medium with 0.5 mg/l TDZ contained the highest level of pseudojujubogenin glycosides [(30.62 ± 1.29) mg/g dry wt] which was 2-fold higher than that of in vitro grown plants of the same age [(16.96 ± 1.49) mg/g dry wt]. Plantlets regenerated from 0.1 mg/l TDZ also showed a high level of pseudojujubogenin glycosides [(27.94 ± 1.19) mg/g dry wt]. The effect of elicitor on pseudojujubogenin glycosides accumulation in B. monnieri whole plant cultures was investigated. Chitosan at 150 mg/l and yeast extract at 2 mg/ml increased the pseudojujubogenin glycosides production [(40.83 ± 2.24) mg/g dry wt and (40.05 ± 2.37) mg/g dry wt, respectively] after 7 days, which was 6-fold higher than in the control cultures.

Original languageEnglish
Pages (from-to)879-883
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume63
Issue number11-12
Publication statusPublished - Dec 1 2008

Fingerprint

Bacopa
Glycosides
Plant Growth Regulators
Chitosan
Yeast
Regeneration
Yeasts
3-O-(beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl) pseudojujubogenin
thidiazuron

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Improvement of pseudojujubogenin glycosides production from regenerated Bacopa monnieri (L.) Wettst. and enhanced yield by elicitors. / Kamonwannasit, Sirilak; Phrompittayarat, Watoo; Ingkaninan, Kornkanok; Tanaka, Hiroyuki; Putalun, Waraporn.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 63, No. 11-12, 01.12.2008, p. 879-883.

Research output: Contribution to journalArticle

Kamonwannasit, Sirilak ; Phrompittayarat, Watoo ; Ingkaninan, Kornkanok ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Improvement of pseudojujubogenin glycosides production from regenerated Bacopa monnieri (L.) Wettst. and enhanced yield by elicitors. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2008 ; Vol. 63, No. 11-12. pp. 879-883.
@article{d996fce04f5d4b52b4851a5a5a2a7494,
title = "Improvement of pseudojujubogenin glycosides production from regenerated Bacopa monnieri (L.) Wettst. and enhanced yield by elicitors",
abstract = "Bacopa monnieri (L.) Wettst. was studied for shoot induction and regeneration on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Stem explants cultured on medium containing 0.1 mg/l thidiazuron (TDZ) resulted in the highest number of shoots (117 shoots/explant). Regenerated plants from medium with 0.5 mg/l TDZ contained the highest level of pseudojujubogenin glycosides [(30.62 ± 1.29) mg/g dry wt] which was 2-fold higher than that of in vitro grown plants of the same age [(16.96 ± 1.49) mg/g dry wt]. Plantlets regenerated from 0.1 mg/l TDZ also showed a high level of pseudojujubogenin glycosides [(27.94 ± 1.19) mg/g dry wt]. The effect of elicitor on pseudojujubogenin glycosides accumulation in B. monnieri whole plant cultures was investigated. Chitosan at 150 mg/l and yeast extract at 2 mg/ml increased the pseudojujubogenin glycosides production [(40.83 ± 2.24) mg/g dry wt and (40.05 ± 2.37) mg/g dry wt, respectively] after 7 days, which was 6-fold higher than in the control cultures.",
author = "Sirilak Kamonwannasit and Watoo Phrompittayarat and Kornkanok Ingkaninan and Hiroyuki Tanaka and Waraporn Putalun",
year = "2008",
month = "12",
day = "1",
language = "English",
volume = "63",
pages = "879--883",
journal = "Zeitschrift fur Naturforschung - Section C Journal of Biosciences",
issn = "0939-5075",
publisher = "Verlag der Zeitschrift fur Naturforschung",
number = "11-12",

}

TY - JOUR

T1 - Improvement of pseudojujubogenin glycosides production from regenerated Bacopa monnieri (L.) Wettst. and enhanced yield by elicitors

AU - Kamonwannasit, Sirilak

AU - Phrompittayarat, Watoo

AU - Ingkaninan, Kornkanok

AU - Tanaka, Hiroyuki

AU - Putalun, Waraporn

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Bacopa monnieri (L.) Wettst. was studied for shoot induction and regeneration on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Stem explants cultured on medium containing 0.1 mg/l thidiazuron (TDZ) resulted in the highest number of shoots (117 shoots/explant). Regenerated plants from medium with 0.5 mg/l TDZ contained the highest level of pseudojujubogenin glycosides [(30.62 ± 1.29) mg/g dry wt] which was 2-fold higher than that of in vitro grown plants of the same age [(16.96 ± 1.49) mg/g dry wt]. Plantlets regenerated from 0.1 mg/l TDZ also showed a high level of pseudojujubogenin glycosides [(27.94 ± 1.19) mg/g dry wt]. The effect of elicitor on pseudojujubogenin glycosides accumulation in B. monnieri whole plant cultures was investigated. Chitosan at 150 mg/l and yeast extract at 2 mg/ml increased the pseudojujubogenin glycosides production [(40.83 ± 2.24) mg/g dry wt and (40.05 ± 2.37) mg/g dry wt, respectively] after 7 days, which was 6-fold higher than in the control cultures.

AB - Bacopa monnieri (L.) Wettst. was studied for shoot induction and regeneration on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Stem explants cultured on medium containing 0.1 mg/l thidiazuron (TDZ) resulted in the highest number of shoots (117 shoots/explant). Regenerated plants from medium with 0.5 mg/l TDZ contained the highest level of pseudojujubogenin glycosides [(30.62 ± 1.29) mg/g dry wt] which was 2-fold higher than that of in vitro grown plants of the same age [(16.96 ± 1.49) mg/g dry wt]. Plantlets regenerated from 0.1 mg/l TDZ also showed a high level of pseudojujubogenin glycosides [(27.94 ± 1.19) mg/g dry wt]. The effect of elicitor on pseudojujubogenin glycosides accumulation in B. monnieri whole plant cultures was investigated. Chitosan at 150 mg/l and yeast extract at 2 mg/ml increased the pseudojujubogenin glycosides production [(40.83 ± 2.24) mg/g dry wt and (40.05 ± 2.37) mg/g dry wt, respectively] after 7 days, which was 6-fold higher than in the control cultures.

UR - http://www.scopus.com/inward/record.url?scp=58849105442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58849105442&partnerID=8YFLogxK

M3 - Article

C2 - 19227838

AN - SCOPUS:58849105442

VL - 63

SP - 879

EP - 883

JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

SN - 0939-5075

IS - 11-12

ER -