Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures

Jukrapun Komaikul, Tharita Kitisripanya, Kittisak Likhitwitayawuid, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

Abstract

Mulberroside A, oxyresveratrol and resveratrol, commonly found in Morus alba L., are potent anti-aging phytostilbenes. In this study, the effect of the addition of 2-hydroxypropyl-β-cyclodextrin on the levels of phytostilbenes in M. alba callus cultures was investigated. Commercial cyclodextrin was used in the hydrolytic and culture processes of the M. alba callus cultures. The hydrolytic study indicated that 2-hydroxypropyl-β-cyclodextrin acted as a retardant for stilbenoid hydrolysis. It reduced mulberroside A deglycosylation and stabilised oxyresveratrol. The elicitation result showed that extracellular oxyresveratrol was increased by adding 2-hydroxypropyl-β-cyclodextrin to the culture media of both free and immobilised M. alba callus (>730-fold and >169-fold, respectively) compared with those of the control. However, the intracellular mulberroside A levels in the treatment groups did not increase compared with those of the control. The results show that the addition of 2-hydroxypropyl-β-cyclodextrin significantly changed the patterns and levels of the stilbenoids in M. alba callus cultures.

Original languageEnglish
Pages (from-to)2762-2769
Number of pages8
JournalNatural Product Research
Volume33
Issue number19
DOIs
Publication statusPublished - Oct 2 2019

Fingerprint

stilbenoids
Morus
Morus alba
cyclodextrins
Bony Callus
Cyclodextrins
callus culture
resveratrol
Culture Media
Hydrolysis
callus
culture media
Aging of materials
hydrolysis
mulberroside A
puag-haad

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

Cite this

Komaikul, J., Kitisripanya, T., Likhitwitayawuid, K., Sritularak, B., Tanaka, H., & Putalun, W. (2019). Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures. Natural Product Research, 33(19), 2762-2769. https://doi.org/10.1080/14786419.2018.1499643

Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures. / Komaikul, Jukrapun; Kitisripanya, Tharita; Likhitwitayawuid, Kittisak; Sritularak, Boonchoo; Tanaka, Hiroyuki; Putalun, Waraporn.

In: Natural Product Research, Vol. 33, No. 19, 02.10.2019, p. 2762-2769.

Research output: Contribution to journalArticle

Komaikul, J, Kitisripanya, T, Likhitwitayawuid, K, Sritularak, B, Tanaka, H & Putalun, W 2019, 'Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures', Natural Product Research, vol. 33, no. 19, pp. 2762-2769. https://doi.org/10.1080/14786419.2018.1499643
Komaikul, Jukrapun ; Kitisripanya, Tharita ; Likhitwitayawuid, Kittisak ; Sritularak, Boonchoo ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures. In: Natural Product Research. 2019 ; Vol. 33, No. 19. pp. 2762-2769.
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