Improvement of the signal-to-noise ratio on the fluorescent detection of a membrane protein using horse radish peroxidase

Takeshi Mori, Kenta Kamino, Tsuyoshi Oda, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

Abstract

Horseradish peroxidase (HRP) is used as a sensitizing enzyme to detect a membrane protein with high sensitivity. In this method, the membrane protein is labeled with HRP, and fluorescent group-modified tyramide is used as a substrate for fluorescent signal amplification. However, a high background signal due to a nonspecific adsorption of fluorescent group- modified tyramide to cells is known to compromise high-sensitive detection. Here, we designed a new substrate in which tyramide was changed to tyrosyltaurine. Because the background signal was greatly reduced by the sulfonate group in the new substrate, the signal- to-noise ratio was greatly improved compared with a commercial substrate.

Original languageEnglish
Pages (from-to)961-964
Number of pages4
Journalbunseki kagaku
Volume68
Issue number12
DOIs
Publication statusPublished - Jan 1 2019

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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