In-cell covalent labeling of reactive his-tag fused proteins

Shohei Uchinomiya, Hiroshi Nonaka, Sho Wakayama, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(ii)-NTA containing an α-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.

Original languageEnglish
Pages (from-to)5022-5024
Number of pages3
JournalChemical Communications
Volume49
Issue number44
DOIs
Publication statusPublished - May 7 2013

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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