TY - JOUR
T1 - In-situ chemical analyses of trans-polyisoprene by histochemical staining and Fourier transform infrared microspectroscopy in a rubber-producing plant, Eucommia ulmoides Oliver
AU - Bamba, Takeshi
AU - Fukusaki, Ei Ichiro
AU - Nakazawa, Yoshihisa
AU - Kobayashi, Akio
N1 - Funding Information:
Acknowledgements This work was supported by the New Energy and Industrial Technology Development Organization (NEDO). The authors are very grateful to Mr. A. Nara (Thermo Nicolet Japan Inc., Toyonaka, Japan) for FT–IR microspectroscopy analyses, Mr. T. Abo (Nihon Microtome Laboratory, Osaka, Japan) and Mr. T. Sando (Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan) for the preparation of tissue sections, and to Prof. A. Shinmyo (Graduate school of biological sciences, Nara Institute of Science and Technology, Ikoma, Japan), Prof. T. Seki, Mr. S. Kajiyama, and Dr. A. Okazawa (Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan) for helpful discussions.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - The localization of polyisoprene in young stem tissues of Eucommia ulmoides Oliver was investigated by histochemical staining and Fourier transform infrared (FT-IR) microspectroscopy. The fibrous structures were stained with Oil Red O. FT-IR microspectroscopic analysis proved that the fibrous structures were trans-polyisoprene. Granular structures stained with the dye, and characteristic absorptions at 2,960 cm-1 and 1,430 cm-1 in FT-IR suggested that trans-polyisoprene accumulated in the vicinity of the cambium layer. We have thus successfully shown for the first time the localization of trans-polyisoprene in plant tissues, and our histological investigation allowed us to presume the main sites of biosynthesis and accumulation of trans-rubber. Furthermore, a new technical approach, the preparation of sections using an electronic freezing unit and the in situ analysis of polyisoprene using FT-IR microspectroscopy, is demonstrated to be a promising method for determining the accumulation of polyisoprene as well as other metabolites.
AB - The localization of polyisoprene in young stem tissues of Eucommia ulmoides Oliver was investigated by histochemical staining and Fourier transform infrared (FT-IR) microspectroscopy. The fibrous structures were stained with Oil Red O. FT-IR microspectroscopic analysis proved that the fibrous structures were trans-polyisoprene. Granular structures stained with the dye, and characteristic absorptions at 2,960 cm-1 and 1,430 cm-1 in FT-IR suggested that trans-polyisoprene accumulated in the vicinity of the cambium layer. We have thus successfully shown for the first time the localization of trans-polyisoprene in plant tissues, and our histological investigation allowed us to presume the main sites of biosynthesis and accumulation of trans-rubber. Furthermore, a new technical approach, the preparation of sections using an electronic freezing unit and the in situ analysis of polyisoprene using FT-IR microspectroscopy, is demonstrated to be a promising method for determining the accumulation of polyisoprene as well as other metabolites.
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U2 - 10.1007/s00425-002-0832-3
DO - 10.1007/s00425-002-0832-3
M3 - Article
C2 - 12355153
AN - SCOPUS:0036942532
SN - 0032-0935
VL - 215
SP - 934
EP - 939
JO - Planta
JF - Planta
IS - 6
ER -