In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar

Ming Xie, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Kengo Nagata, Yukiko Ookuma, Hiroaki Fujiwara, Hidetaka Sakai

Research output: Contribution to journalArticle

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Abstract

We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially expressed genes in the E12.0 mandible. This study examined the precise expression pattern of Rpl21 mRNA in the mouse mandibular first molar by in situ hybridization. Rpl21 mRNA was expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0. Strong in situ signals were observed in the epithelial bud at E14.0, and in the enamel organ at E15.0. However, either no (E14.0) or only a weak (E15.0) in situ signal was found in the primary enamel knot at these gestational days. Rpl21 was strongly expressed in the innerenamel epithelium, cervical loop and dental lamina from E16.0 to E18.0. In addition, Rpl21 mRNA was also demonstrated in various developing cranio-facial organs. These results suggest that Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of such tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast. Rpl21 for protein synthesis might also be related to the morphogenesis of the developing cranio-facial organs.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalJournal of Molecular Histology
Volume40
Issue number5-6
DOIs
Publication statusPublished - Oct 1 2009

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Tooth Germ
Odontogenesis
Tooth
Epithelium
Dental Enamel
Mandible
Messenger RNA
Enamel Organ
Ameloblasts
Mesoderm
Regulator Genes
ribosomal protein L21
Morphogenesis
In Situ Hybridization
Complementary DNA
Peptides

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Histology
  • Physiology

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In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar. / Xie, Ming; Kobayashi, Ieyoshi; Kiyoshima, Tamotsu; Nagata, Kengo; Ookuma, Yukiko; Fujiwara, Hiroaki; Sakai, Hidetaka.

In: Journal of Molecular Histology, Vol. 40, No. 5-6, 01.10.2009, p. 361-367.

Research output: Contribution to journalArticle

Xie, Ming ; Kobayashi, Ieyoshi ; Kiyoshima, Tamotsu ; Nagata, Kengo ; Ookuma, Yukiko ; Fujiwara, Hiroaki ; Sakai, Hidetaka. / In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar. In: Journal of Molecular Histology. 2009 ; Vol. 40, No. 5-6. pp. 361-367.
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