In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar

Jun Ya Honda, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Kengo Nagata, Hiroko Wada, Yukiko Ookuma, Hiroaki Fujiwara, Maho Shiotsuka, Ichiro Takahashi, Hidetaka Sakai

Research output: Contribution to journalArticle

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Abstract

We previously performed cDNA subtraction between the mouse mandibles on embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to identify genes expressed at its beginning. Adenosine triphosphate synthase subunit a (Atpase6) is one of the highly expressed genes in the E12.0 mandible including tooth germs. In situ hybridization was conducted using the mouse mandibular first molar from E10.5 to E18.0 to determine the precise expression patterns of Atpase6 mRNA in the developing tooth germ. Atpase6 mRNA was strongly expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0 and E13.0. Strong in situ signals were observed in the epithelium at E14.0, and in the enamel organ excluded the area of the primary enamel knot at E15.0. Atpase6 was strongly expressed in the inner enamel epithelium, the adjacent stratum intermedium, and the outer enamel epithelium in the cervical loops from E16.0 to E18.0. In addition, strong Atpase6 signals were coincidently demonstrated in various developing cranio-facial organs. These results suggest that Atpase6 participates in the high energy-utilizing functions of the cells related to the initiation and the development of the tooth germ as well as those of the other cranio-facial organs.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalJournal of Molecular Histology
Volume42
Issue number1
DOIs
Publication statusPublished - Feb 1 2011

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Tooth Germ
Mitochondrial Genes
Epithelium
Dental Enamel
Mandible
Tooth
Odontogenesis
Enamel Organ
Messenger RNA
Mesoderm
Genes
In Situ Hybridization
Complementary DNA
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Histology
  • Physiology

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In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar. / Honda, Jun Ya; Kobayashi, Ieyoshi; Kiyoshima, Tamotsu; Nagata, Kengo; Wada, Hiroko; Ookuma, Yukiko; Fujiwara, Hiroaki; Shiotsuka, Maho; Takahashi, Ichiro; Sakai, Hidetaka.

In: Journal of Molecular Histology, Vol. 42, No. 1, 01.02.2011, p. 83-90.

Research output: Contribution to journalArticle

Honda, Jun Ya ; Kobayashi, Ieyoshi ; Kiyoshima, Tamotsu ; Nagata, Kengo ; Wada, Hiroko ; Ookuma, Yukiko ; Fujiwara, Hiroaki ; Shiotsuka, Maho ; Takahashi, Ichiro ; Sakai, Hidetaka. / In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar. In: Journal of Molecular Histology. 2011 ; Vol. 42, No. 1. pp. 83-90.
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AU - Wada, Hiroko

AU - Ookuma, Yukiko

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