TY - JOUR
T1 - In situ-formed, tissue-adhesive co-gel composed of styrenated gelatin and styrenated antibody
T2 - Potential use for local anti-cytokine antibody therapy on surgically resected tissues
AU - Manabe, Tatsuya
AU - Okino, Hidenobu
AU - Tanaka, Masao
AU - Matsuda, Takehisa
N1 - Funding Information:
This study is financially supported by Promotion Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (OPSR, contract grant number: 97-15) and Grant-in-Aid for Scientific Research from Ministry of Education, Culture, Sports, Science, and Technology of Japan (contract grant number: A2-12358017 and B2-12470277). The authors wish to thank K. Yasutake for excellent technical assistance.
PY - 2004/12
Y1 - 2004/12
N2 - Styrenated antibody (ST-Ab) and styrenated gelatin (ST-gelatin) were prepared by condensation reaction of antibody or gelatin with 4-vinylbenzoic acid, respectively. The affinity loss of ST-Ab to its antigen was minimal. ST-Ab and ST-gelatin were copolymerized with by visible-light irradiation in the presence of a water-soluble camphorquinone as a photoinitiator to produce a tissue-adhesive, in situ-formed co-gel of ST-gelatin and ST-Ab. The amount of non-reacted ST-Ab released from the co-gel of ST-gelatin and ST-Ab into the medium was minimal. The confocal laser scanning microscopy observation showed that local accumulation of rhodamine-labeled bovine serum albumin (BSA) as a model antigen was noticed in the surface-to-subsurface region of the co-gel of ST-gelatin and anti-BSA ST-Ab, indicating that the gel prevented the permeation of BSA into the gel. In invasion double chamber assay using anti-hepatocyte growth factor (HGF) antibody, the co-gel prevented HGF-dependent invasion of pancreatic cancer cells. The discussion was made for potential application of an in situ-formed tissue-adhesive co-gel of ST-gelatin and ST-Ab, developed in this study, as a cytokine-barrier on a surgically resected tissue where cancer cells might still remain after resection of cancerous tissue.
AB - Styrenated antibody (ST-Ab) and styrenated gelatin (ST-gelatin) were prepared by condensation reaction of antibody or gelatin with 4-vinylbenzoic acid, respectively. The affinity loss of ST-Ab to its antigen was minimal. ST-Ab and ST-gelatin were copolymerized with by visible-light irradiation in the presence of a water-soluble camphorquinone as a photoinitiator to produce a tissue-adhesive, in situ-formed co-gel of ST-gelatin and ST-Ab. The amount of non-reacted ST-Ab released from the co-gel of ST-gelatin and ST-Ab into the medium was minimal. The confocal laser scanning microscopy observation showed that local accumulation of rhodamine-labeled bovine serum albumin (BSA) as a model antigen was noticed in the surface-to-subsurface region of the co-gel of ST-gelatin and anti-BSA ST-Ab, indicating that the gel prevented the permeation of BSA into the gel. In invasion double chamber assay using anti-hepatocyte growth factor (HGF) antibody, the co-gel prevented HGF-dependent invasion of pancreatic cancer cells. The discussion was made for potential application of an in situ-formed tissue-adhesive co-gel of ST-gelatin and ST-Ab, developed in this study, as a cytokine-barrier on a surgically resected tissue where cancer cells might still remain after resection of cancerous tissue.
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U2 - 10.1016/j.biomaterials.2004.01.036
DO - 10.1016/j.biomaterials.2004.01.036
M3 - Article
C2 - 15172499
AN - SCOPUS:2542495798
SN - 0142-9612
VL - 25
SP - 5867
EP - 5873
JO - Biomaterials
JF - Biomaterials
IS - 27
ER -
