In situ-formed, tissue-adhesive co-gel composed of styrenated gelatin and styrenated antibody

Potential use for local anti-cytokine antibody therapy on surgically resected tissues

Tatsuya Manabe, Hidenobu Okino, Masao Tanaka, Takehisa Matsuda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Styrenated antibody (ST-Ab) and styrenated gelatin (ST-gelatin) were prepared by condensation reaction of antibody or gelatin with 4-vinylbenzoic acid, respectively. The affinity loss of ST-Ab to its antigen was minimal. ST-Ab and ST-gelatin were copolymerized with by visible-light irradiation in the presence of a water-soluble camphorquinone as a photoinitiator to produce a tissue-adhesive, in situ-formed co-gel of ST-gelatin and ST-Ab. The amount of non-reacted ST-Ab released from the co-gel of ST-gelatin and ST-Ab into the medium was minimal. The confocal laser scanning microscopy observation showed that local accumulation of rhodamine-labeled bovine serum albumin (BSA) as a model antigen was noticed in the surface-to-subsurface region of the co-gel of ST-gelatin and anti-BSA ST-Ab, indicating that the gel prevented the permeation of BSA into the gel. In invasion double chamber assay using anti-hepatocyte growth factor (HGF) antibody, the co-gel prevented HGF-dependent invasion of pancreatic cancer cells. The discussion was made for potential application of an in situ-formed tissue-adhesive co-gel of ST-gelatin and ST-Ab, developed in this study, as a cytokine-barrier on a surgically resected tissue where cancer cells might still remain after resection of cancerous tissue.

Original languageEnglish
Pages (from-to)5867-5873
Number of pages7
JournalBiomaterials
Volume25
Issue number27
DOIs
Publication statusPublished - Dec 1 2004

Fingerprint

Tissue Adhesives
Gelatin
Antibodies
Anti-Idiotypic Antibodies
Adhesives
Gels
Tissue
Cytokines
Bovine Serum Albumin
Therapeutics
Hepatocyte Growth Factor
Antigens
Cells
Rhodamines
Condensation reactions
Pancreatic Neoplasms
Confocal Microscopy
Permeation
Assays
Microscopic examination

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

In situ-formed, tissue-adhesive co-gel composed of styrenated gelatin and styrenated antibody : Potential use for local anti-cytokine antibody therapy on surgically resected tissues. / Manabe, Tatsuya; Okino, Hidenobu; Tanaka, Masao; Matsuda, Takehisa.

In: Biomaterials, Vol. 25, No. 27, 01.12.2004, p. 5867-5873.

Research output: Contribution to journalArticle

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