In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging

Yoon Hee Kim, Yoshinori Fujimura, Masako Sasaki, Xue Yang, Daichi Yukihira, Daisuke Miura, Yumi Unno, Koretsugu Ogata, Hiroki Nakajima, Shuya Yamashita, Kanami Nakahara, Motoki Murata, I. Chian Lin, Hiroyuki Wariishi, Koji Yamada, Hirofumi Tachibana

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.

Original languageEnglish
Pages (from-to)9279-9285
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number38
DOIs
Publication statusPublished - Sep 24 2014

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matrix-assisted laser desorption-ionization mass spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Labels
Visualization
kidneys
image analysis
Kidney
Imaging techniques
mice
Ionization
Mass spectrometry
Desorption
ferulic acid
Lasers
green tea
methodology
Biomolecules
Tea
mouth
strictinin

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging. / Kim, Yoon Hee; Fujimura, Yoshinori; Sasaki, Masako; Yang, Xue; Yukihira, Daichi; Miura, Daisuke; Unno, Yumi; Ogata, Koretsugu; Nakajima, Hiroki; Yamashita, Shuya; Nakahara, Kanami; Murata, Motoki; Lin, I. Chian; Wariishi, Hiroyuki; Yamada, Koji; Tachibana, Hirofumi.

In: Journal of Agricultural and Food Chemistry, Vol. 62, No. 38, 24.09.2014, p. 9279-9285.

Research output: Contribution to journalArticle

Kim, YH, Fujimura, Y, Sasaki, M, Yang, X, Yukihira, D, Miura, D, Unno, Y, Ogata, K, Nakajima, H, Yamashita, S, Nakahara, K, Murata, M, Lin, IC, Wariishi, H, Yamada, K & Tachibana, H 2014, 'In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging', Journal of Agricultural and Food Chemistry, vol. 62, no. 38, pp. 9279-9285. https://doi.org/10.1021/jf503143g
Kim, Yoon Hee ; Fujimura, Yoshinori ; Sasaki, Masako ; Yang, Xue ; Yukihira, Daichi ; Miura, Daisuke ; Unno, Yumi ; Ogata, Koretsugu ; Nakajima, Hiroki ; Yamashita, Shuya ; Nakahara, Kanami ; Murata, Motoki ; Lin, I. Chian ; Wariishi, Hiroyuki ; Yamada, Koji ; Tachibana, Hirofumi. / In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging. In: Journal of Agricultural and Food Chemistry. 2014 ; Vol. 62, No. 38. pp. 9279-9285.
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