In situ localization of CD83-positive dendritic cells in psoriatic lesions

Tetsuya Koga, Hong Duan, Kazunori Urabe, Masutaka Furue

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Dendritic cells (DC) are considered to be the most potent antigen-presenting cells, and CD83 is expressed at a high level on immune-competent, activated and mature DC. Although changes in the number or localization of mature and activated CD83+ DC could be expected in psoriasis, there is little information on such changes. Aim: Morphological identification of CD83+ DC in psoriatic skin lesions. Materials and Methods: Immunohistochemical staining was performed in 5 specimens of psoriasis vulgaris and 6 specimens of pustular psoriasis. Formalin-fixed, paraffin-embedded sections were used for examination in this study. The skin sections were pretreated with 0.1% trypsin for 60 min at 37°C prior to immunostaining for CD83. Results: A small but significant subpopulation of CD83+ DC was found in the upper dermis. In addition, CD83+ DC were occasionally scattered in the epidermis. The most common distribution pattern of CD83+ DC was as clusters with mononuclear lymphoid cells in the upper dermis. CD83+ DC were in close contact with lymphocytes. High-intensity staining of CD83 antigens was detected not only on the surface, but also in the cytoplasm of DC. Conclusion: These results indicate that activated and mature CD83+ DC may play a role in the immune response in psoriasis and provide in vivo support for the concept that CD83+ DC provide signals for direct intralesional T cell activation.

Original languageEnglish
Pages (from-to)100-103
Number of pages4
JournalDermatology
Volume204
Issue number2
DOIs
Publication statusPublished - Apr 22 2002

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Dendritic Cells
Psoriasis
Dermis
Lymphocytes
Staining and Labeling
Skin
Antigen-Presenting Cells
Epidermis
Paraffin
Trypsin
Formaldehyde
Cytoplasm
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

In situ localization of CD83-positive dendritic cells in psoriatic lesions. / Koga, Tetsuya; Duan, Hong; Urabe, Kazunori; Furue, Masutaka.

In: Dermatology, Vol. 204, No. 2, 22.04.2002, p. 100-103.

Research output: Contribution to journalArticle

Koga, Tetsuya ; Duan, Hong ; Urabe, Kazunori ; Furue, Masutaka. / In situ localization of CD83-positive dendritic cells in psoriatic lesions. In: Dermatology. 2002 ; Vol. 204, No. 2. pp. 100-103.
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