In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device

Do Hyeon Yang, Ah Hyun Bae, Kazuya Koumoto, Seung Woo Lee, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Schizophyllan belongs to the β-(1 → 3)-d-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through a long methylene spacer was synthesized, and its interaction with polynucleotides was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K+ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules.

Original languageEnglish
Pages (from-to)490-494
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume105
Issue number2
DOIs
Publication statusPublished - Mar 28 2005

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

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