In situ observation of spherical DNA assembly in water and the controlled release of bound dyes

Kazunori Matsuura, Kouzo Masumoto, Yuuko Igami, Tatsuro Fujioka, Nobuo Kimizuka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Three strands of 30-mer oligodeoxyribonucleotides (ODNs) were designed to form three-way junctions that possess self-complementary sticky ends. The morphology of self-assembled ODNs in water was observed in situ by confocal laser scanning fluorescence microscopy. The three-way junctions self-assembled into spherical assemblies, in accordance with transmission and scanning electron microscopy. The size of nucleospheres was in the range of several tens of nanometers to micrometers, which varied depending on the concentration of ODNs and added salts. Fluorescence images of spherical ODN assemblies suggested that the nucleospheres possess multiwalled structures. The fluorescence of sodium 1-anilinonaphthalene-8-sulfonate in the presence of nucleospheres revealed that the interior of nucleospheres possesses polarity corresponding to that between methanol and ethanol. A dye-inclusion experiment showed that cationic and even anionic dyes were adsorbed to the interior of the nucleospheres. The dye-included nucleospheres released dyes by thermal dissociation or digestion of the constituent ODNs.

Original languageEnglish
Pages (from-to)2726-2732
Number of pages7
JournalBiomacromolecules
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 1 2007

Fingerprint

Oligodeoxyribonucleotides
DNA
Coloring Agents
Dyes
Observation
Water
Fluorescence
Scanning Transmission Electron Microscopy
Fluorescence microscopy
Fluorescence Microscopy
Confocal Microscopy
Methanol
Digestion
Ethanol
Salts
Hot Temperature
Sodium
Transmission electron microscopy
Scanning
Scanning electron microscopy

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

In situ observation of spherical DNA assembly in water and the controlled release of bound dyes. / Matsuura, Kazunori; Masumoto, Kouzo; Igami, Yuuko; Fujioka, Tatsuro; Kimizuka, Nobuo.

In: Biomacromolecules, Vol. 8, No. 9, 01.09.2007, p. 2726-2732.

Research output: Contribution to journalArticle

Matsuura, Kazunori ; Masumoto, Kouzo ; Igami, Yuuko ; Fujioka, Tatsuro ; Kimizuka, Nobuo. / In situ observation of spherical DNA assembly in water and the controlled release of bound dyes. In: Biomacromolecules. 2007 ; Vol. 8, No. 9. pp. 2726-2732.
@article{5bf521dd7bc4402a841a8f0718df6ebc,
title = "In situ observation of spherical DNA assembly in water and the controlled release of bound dyes",
abstract = "Three strands of 30-mer oligodeoxyribonucleotides (ODNs) were designed to form three-way junctions that possess self-complementary sticky ends. The morphology of self-assembled ODNs in water was observed in situ by confocal laser scanning fluorescence microscopy. The three-way junctions self-assembled into spherical assemblies, in accordance with transmission and scanning electron microscopy. The size of nucleospheres was in the range of several tens of nanometers to micrometers, which varied depending on the concentration of ODNs and added salts. Fluorescence images of spherical ODN assemblies suggested that the nucleospheres possess multiwalled structures. The fluorescence of sodium 1-anilinonaphthalene-8-sulfonate in the presence of nucleospheres revealed that the interior of nucleospheres possesses polarity corresponding to that between methanol and ethanol. A dye-inclusion experiment showed that cationic and even anionic dyes were adsorbed to the interior of the nucleospheres. The dye-included nucleospheres released dyes by thermal dissociation or digestion of the constituent ODNs.",
author = "Kazunori Matsuura and Kouzo Masumoto and Yuuko Igami and Tatsuro Fujioka and Nobuo Kimizuka",
year = "2007",
month = "9",
day = "1",
doi = "10.1021/bm070357w",
language = "English",
volume = "8",
pages = "2726--2732",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - In situ observation of spherical DNA assembly in water and the controlled release of bound dyes

AU - Matsuura, Kazunori

AU - Masumoto, Kouzo

AU - Igami, Yuuko

AU - Fujioka, Tatsuro

AU - Kimizuka, Nobuo

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Three strands of 30-mer oligodeoxyribonucleotides (ODNs) were designed to form three-way junctions that possess self-complementary sticky ends. The morphology of self-assembled ODNs in water was observed in situ by confocal laser scanning fluorescence microscopy. The three-way junctions self-assembled into spherical assemblies, in accordance with transmission and scanning electron microscopy. The size of nucleospheres was in the range of several tens of nanometers to micrometers, which varied depending on the concentration of ODNs and added salts. Fluorescence images of spherical ODN assemblies suggested that the nucleospheres possess multiwalled structures. The fluorescence of sodium 1-anilinonaphthalene-8-sulfonate in the presence of nucleospheres revealed that the interior of nucleospheres possesses polarity corresponding to that between methanol and ethanol. A dye-inclusion experiment showed that cationic and even anionic dyes were adsorbed to the interior of the nucleospheres. The dye-included nucleospheres released dyes by thermal dissociation or digestion of the constituent ODNs.

AB - Three strands of 30-mer oligodeoxyribonucleotides (ODNs) were designed to form three-way junctions that possess self-complementary sticky ends. The morphology of self-assembled ODNs in water was observed in situ by confocal laser scanning fluorescence microscopy. The three-way junctions self-assembled into spherical assemblies, in accordance with transmission and scanning electron microscopy. The size of nucleospheres was in the range of several tens of nanometers to micrometers, which varied depending on the concentration of ODNs and added salts. Fluorescence images of spherical ODN assemblies suggested that the nucleospheres possess multiwalled structures. The fluorescence of sodium 1-anilinonaphthalene-8-sulfonate in the presence of nucleospheres revealed that the interior of nucleospheres possesses polarity corresponding to that between methanol and ethanol. A dye-inclusion experiment showed that cationic and even anionic dyes were adsorbed to the interior of the nucleospheres. The dye-included nucleospheres released dyes by thermal dissociation or digestion of the constituent ODNs.

UR - http://www.scopus.com/inward/record.url?scp=34948827539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34948827539&partnerID=8YFLogxK

U2 - 10.1021/bm070357w

DO - 10.1021/bm070357w

M3 - Article

VL - 8

SP - 2726

EP - 2732

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 9

ER -