In Situ Spectroscopic Quantification of Protein-Ice Interactions

Alan Twomey, Rebekah Less, Kosaku Kurata, Hiroshi Takamatsu, Alptekin Aksan

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

FTIR and confocal Raman microspectroscopy were used to measure interactions between albumin and ice in situ during quasi-equilibrium freezing in dimethyl sulfoxide (DMSO) solutions. At temperatures of -4 and -6 C, albumin was found to be preferentially excluded from the ice phase during near-equilibrium freezing. This behavior reversed at lower temperatures. Instead, DMSO was preferentially excluded from the ice phase, resulting in an albumin concentration in the freeze-concentrated liquid phase that was lower than predicted. It is hypothesized that this was caused by the albumin in the freeze-concentrated liquid getting adsorbed onto the ice surface or becoming entrapped in the ice phase. It was observed that, under certain freezing protocols, as much as 20% of the albumin in solutions with starting concentrations of 32-53 mg/mL may be adsorbed onto the ice interface or entrapped in the ice phase.

Original languageEnglish
Pages (from-to)7889-7897
Number of pages9
JournalJournal of Physical Chemistry B
Volume117
Issue number26
DOIs
Publication statusPublished - Jul 3 2013

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Ice
ice
albumins
proteins
Proteins
Albumins
Freezing
freezing
interactions
Dimethyl sulfoxide
Dimethyl Sulfoxide
Temperature
Liquids
Fourier Transform Infrared Spectroscopy
liquid phases
liquids

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

In Situ Spectroscopic Quantification of Protein-Ice Interactions. / Twomey, Alan; Less, Rebekah; Kurata, Kosaku; Takamatsu, Hiroshi; Aksan, Alptekin.

In: Journal of Physical Chemistry B, Vol. 117, No. 26, 03.07.2013, p. 7889-7897.

Research output: Contribution to journalArticle

Twomey, Alan ; Less, Rebekah ; Kurata, Kosaku ; Takamatsu, Hiroshi ; Aksan, Alptekin. / In Situ Spectroscopic Quantification of Protein-Ice Interactions. In: Journal of Physical Chemistry B. 2013 ; Vol. 117, No. 26. pp. 7889-7897.
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