In situ studies on protein adsorption onto a poly(2-methoxyethylacrylate) surface by a quartz crystal microbalance

Masaru Tanaka, Akira Mochizuki, Tadahiro Motomura, Kenichi Shimura, Makoto Onishi, Yoshio Okahata

Research output: Contribution to journalArticle

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Abstract

We have reported that poly(2-methoxyethylacrylate) (PMEA) showed excellent blood compatibility though it had a simple chemical structure, and have been making efforts to clarify the reason for the blood compatibility. It is well-known that the adsorption behavior of the protein affects the compatibility. Therefore, the adsorption behaviors of bovine serum albumin (BSA) and human fibrinogen onto the surfaces of PMEA, poly(2-hydroxyethyl methacrylate) (PHEMA) and polypropylene (PP) were investigated by using a quartz crystal microbalance (QCM), where PHEMA and PP were selected as the representatives of hydrophilic and hydrophobic polymers, respectively. Both proteins were observed to adsorb onto all the polymer surfaces according to Langmuir's adsorption isotherm. The maximum adsorption amounts and the apparent association constants of the proteins for PMEA obtained from the isotherm were lower than those for PHEMA and PP. These results suggest that the interaction between PMEA and the proteins is weaker than the interaction with PP and PHEMA.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume193
Issue number1-3
DOIs
Publication statusPublished - Dec 15 2001

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Polypropylenes
Quartz crystal microbalances
quartz crystals
polypropylene
microbalances
compatibility
proteins
Proteins
Adsorption
adsorption
blood
Polymers
isotherms
Blood
fibrinogen
polymers
Bovine Serum Albumin
albumins
Adsorption isotherms
serums

All Science Journal Classification (ASJC) codes

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

In situ studies on protein adsorption onto a poly(2-methoxyethylacrylate) surface by a quartz crystal microbalance. / Tanaka, Masaru; Mochizuki, Akira; Motomura, Tadahiro; Shimura, Kenichi; Onishi, Makoto; Okahata, Yoshio.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 193, No. 1-3, 15.12.2001, p. 145-152.

Research output: Contribution to journalArticle

Tanaka, Masaru ; Mochizuki, Akira ; Motomura, Tadahiro ; Shimura, Kenichi ; Onishi, Makoto ; Okahata, Yoshio. / In situ studies on protein adsorption onto a poly(2-methoxyethylacrylate) surface by a quartz crystal microbalance. In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2001 ; Vol. 193, No. 1-3. pp. 145-152.
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