In vitro binding study of adaptor protein complex (AP-1) to lysosomal targeting motif (LI-motif)

Hideaki Fujita, Masayo Saeki, Kumiko Yasunaga, Tadashi Ueda, Taiji Imoto, Masaru Himeno

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Lysosomal membrane glycoproteins carry targeting information in cytoplasmic regions. Two distinct targeting motifs in these regions, GY (glycine-tyrosine) and LI (leucine-isoleucine), have been identified and characterized. Accumulating evidence suggests that the adaptor complexes (AP-1, AP-2, and AP-3) recognize this information in cytoplasmic tails of transmembrane proteins. Here we report two different in vitro analyses (affinity beads and surface plasmon resonance) which revealed specific interaction between the cytoplasmic tail of LGP85 and AP-1 but not so with AP-2. We also noted requirement of the LI motif of the LGP85 tail in binding to the AP-1 complex. Our data and others which indicated the binding of AP-3 to the LIMP II (synonym of LGP85) tail suggest that the cytoplasmic tail of LGP85 interacts with AP-1 at the trans-Golgi network (TGN) and AP-3 at late endosomes, respectively. We propose that this sequential interaction between the lysosomal targeting signal and distinct APs along its transport pathway is responsible for the critical sorting of lysosomal membrane proteins and/or the potential proofreading system of mistargeted molecules.

Original languageEnglish
Pages (from-to)54-58
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume255
Issue number1
DOIs
Publication statusPublished - Feb 5 1999

Fingerprint

Adaptor Protein Complex 1
Transcription Factor AP-1
Lysosome-Associated Membrane Glycoproteins
Isoleucine
Leucine
Proteins
trans-Golgi Network
Surface Plasmon Resonance
Endosomes
Surface plasmon resonance
Sorting
Glycine
Tyrosine
Molecules
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

In vitro binding study of adaptor protein complex (AP-1) to lysosomal targeting motif (LI-motif). / Fujita, Hideaki; Saeki, Masayo; Yasunaga, Kumiko; Ueda, Tadashi; Imoto, Taiji; Himeno, Masaru.

In: Biochemical and Biophysical Research Communications, Vol. 255, No. 1, 05.02.1999, p. 54-58.

Research output: Contribution to journalArticle

Fujita, Hideaki ; Saeki, Masayo ; Yasunaga, Kumiko ; Ueda, Tadashi ; Imoto, Taiji ; Himeno, Masaru. / In vitro binding study of adaptor protein complex (AP-1) to lysosomal targeting motif (LI-motif). In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 255, No. 1. pp. 54-58.
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