In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium

Hiroyuki Wariishi; Khadar Valli; Michael H. Gold

doi:10.1016/0006-291X(91)90919-X

In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium

Hiroyuki Wariishi, Khadar Valli, Michael H. Gold

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248 Citations (Scopus)

Abstract

Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different ¹⁴C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of ¹⁴C-sidechain-labeled syringyl lignin, a ¹⁴C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of ¹⁴C-sidechain- and ¹⁴C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.

Original language	English
Pages (from-to)	269-275
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(91)90919-X
Publication status	Published - Apr 15 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(91)90919-X

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title = "In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium",

abstract = "Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different 14C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of 14C-sidechain-labeled syringyl lignin, a 14C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of 14C-sidechain- and 14C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.",

author = "Hiroyuki Wariishi and Khadar Valli and Gold, {Michael H.}",

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AU - Wariishi, Hiroyuki

AU - Valli, Khadar

AU - Gold, Michael H.

N1 - Funding Information: by NSF grant

PY - 1991/4/15

Y1 - 1991/4/15

N2 - Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different 14C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of 14C-sidechain-labeled syringyl lignin, a 14C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of 14C-sidechain- and 14C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.

AB - Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different 14C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of 14C-sidechain-labeled syringyl lignin, a 14C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of 14C-sidechain- and 14C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.

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In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium

Abstract

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