In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium

Hiroyuki Wariishi; Khadar Valli; Michael H. Gold

doi:10.1016/0006-291X(91)90919-X

In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium

Hiroyuki Wariishi, Khadar Valli, Michael H. Gold

Research output: Contribution to journal › Article

242 Citations (Scopus)

Abstract

Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different ¹⁴C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of ¹⁴C-sidechain-labeled syringyl lignin, a ¹⁴C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of ¹⁴C-sidechain- and ¹⁴C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.

Original language	English
Pages (from-to)	269-275
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(91)90919-X
Publication status	Published - Apr 15 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(91)90919-X

Fingerprint Dive into the research topics of 'In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium'. Together they form a unique fingerprint.

Cite this

Wariishi, H., Valli, K., & Gold, M. H. (1991). In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium. Biochemical and Biophysical Research Communications, 176(1), 269-275. https://doi.org/10.1016/0006-291X(91)90919-X

@article{28375cc699654d3ebec5d03dc9dc52f1,

title = "In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium",

abstract = "Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different 14C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of 14C-sidechain-labeled syringyl lignin, a 14C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of 14C-sidechain- and 14C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.",

author = "Hiroyuki Wariishi and Khadar Valli and Gold, {Michael H.}",

year = "1991",

month = apr,

day = "15",

doi = "10.1016/0006-291X(91)90919-X",

language = "English",

volume = "176",

pages = "269--275",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium

AU - Wariishi, Hiroyuki

AU - Valli, Khadar

AU - Gold, Michael H.

PY - 1991/4/15

Y1 - 1991/4/15

N2 - Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different 14C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of 14C-sidechain-labeled syringyl lignin, a 14C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of 14C-sidechain- and 14C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.

AB - Homogeneous manganese peroxidase catalyzed the in vitro partial depolymerization of four different 14C-labeled synthetic lignin preparations. Gel permeation profiles demonstrated significant depolymerization of 14C-sidechain-labeled syringyl lignin, a 14C-sidechain-labeled syringyl-guaiacyl copolymer (angiosperm lignin), and depolymerization of 14C-sidechain- and 14C-ring-labeled guaiacyl lignins (gymnosperm lignin). 3,5-Dimethoxy-1,4-benzoquinone, 3,5-dimethoxy-1,4-hydroquinone, and syringylaldehyde were identified as degradation products of the syringyl and syringyl-guaiacyl lignins. These results suggest that manganese peroxidase plays a significant role in the depolymerization of lignin by Phanerochaete chrysosporium.

UR - http://www.scopus.com/inward/record.url?scp=0025769643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025769643&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(91)90919-X

DO - 10.1016/0006-291X(91)90919-X

M3 - Article

C2 - 2018522

AN - SCOPUS:0025769643

VL - 176

SP - 269

EP - 275

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -