In vitro endothelialization test of biomaterials using immortalized endothelial cells

Ken Kono, Hitomi Hiruma, Shingo Kobayashi, Yoji Sato, Masaru Tanaka, Rumi Sawada, Shingo Niimi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. To assess endothelialization of materials in vitro, primary ECs are generally used, although the characteristics of these cells vary among the donors and change with time in culture. Recently, primary cell lines immortalized by transduction of simian vacuolating virus 40 large T antigen or human telomerase reverse transcriptase have been developed. To determine whether immortalized ECs can substitute for primary ECs in material testing, we investigated endothelialization on biocompatible polymers using three lots of primary human umbilical vein endothelial cells (HUVEC) and immortalized microvascular ECs, TIME-GFP. Attachment to and growth on polymer surfaces were comparable between cell types, but results were more consistent with TIME-GFP. Our findings indicate that TIME-GFP is more suitable for in vitro endothelialization testing of biomaterials.

Original languageEnglish
Article numbere0158289
JournalPloS one
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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