In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa; Shunichi Sato; Makoto Ogura; Yoshinori Masaki; Maki Uenoyama; Hitoshi Wakisaka; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.588852

In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

Research output: Contribution to journal › Conference article › peer-review

Abstract

Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm², but at higher fluences (>1.7 J/cm²), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

Original language	English
Article number	36
Pages (from-to)	225-229
Number of pages	5
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5695
DOIs	https://doi.org/10.1117/12.588852
Publication status	Published - Aug 16 2005
Externally published	Yes
Event	Optical Interactions with Tissue and Cells XVI - San Jose, CA, United States Duration: Jan 24 2005 → Jan 26 2005

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.588852

Cite this

In vitro gene transfer by the use of laser-induced stress wave : Effect of laser irradiation conditions on transfection efficiency. / Terakawa, Mitsuhiro; Sato, Shunichi; Ogura, Makoto et al.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5695, 36, 16.08.2005, p. 225-229.

Research output: Contribution to journal › Conference article › peer-review

@article{553e304857bf4355bac939b8f5064847,

title = "In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency",

abstract = "Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.",

author = "Mitsuhiro Terakawa and Shunichi Sato and Makoto Ogura and Yoshinori Masaki and Maki Uenoyama and Hitoshi Wakisaka and Hiroshi Ashida and Minoru Obara",

year = "2005",

month = aug,

day = "16",

doi = "10.1117/12.588852",

language = "English",

volume = "5695",

pages = "225--229",

journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

issn = "1605-7422",

publisher = "SPIE",

note = "Optical Interactions with Tissue and Cells XVI ; Conference date: 24-01-2005 Through 26-01-2005",

}

TY - JOUR

T1 - In vitro gene transfer by the use of laser-induced stress wave

T2 - Optical Interactions with Tissue and Cells XVI

AU - Terakawa, Mitsuhiro

AU - Sato, Shunichi

AU - Ogura, Makoto

AU - Masaki, Yoshinori

AU - Uenoyama, Maki

AU - Wakisaka, Hitoshi

AU - Ashida, Hiroshi

AU - Obara, Minoru

PY - 2005/8/16

Y1 - 2005/8/16

N2 - Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

AB - Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

UR - http://www.scopus.com/inward/record.url?scp=23244456666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23244456666&partnerID=8YFLogxK

U2 - 10.1117/12.588852

DO - 10.1117/12.588852

M3 - Conference article

AN - SCOPUS:23244456666

SN - 1605-7422

VL - 5695

SP - 225

EP - 229

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 36

Y2 - 24 January 2005 through 26 January 2005

ER -

In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this