In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

Research output: Contribution to journalConference article

Abstract

Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

Original languageEnglish
Article number36
Pages (from-to)225-229
Number of pages5
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5695
DOIs
Publication statusPublished - Aug 16 2005
EventOptical Interactions with Tissue and Cells XVI - San Jose, CA, United States
Duration: Jan 24 2005Jan 26 2005

Fingerprint

Gene transfer
Wave effects
stress waves
Laser beam effects
genes
Transfection
Lasers
irradiation
Genes
lasers
fluence
Laser pulses
Gene therapy
gene therapy
Controllability
plasmids
Pulsed lasers
controllability
pulses
Plasmids

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

In vitro gene transfer by the use of laser-induced stress wave : Effect of laser irradiation conditions on transfection efficiency. / Terakawa, Mitsuhiro; Sato, Shunichi; Ogura, Makoto; Masaki, Yoshinori; Uenoyama, Maki; Wakisaka, Hitoshi; Ashida, Hiroshi; Obara, Minoru.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5695, 36, 16.08.2005, p. 225-229.

Research output: Contribution to journalConference article

Terakawa, Mitsuhiro ; Sato, Shunichi ; Ogura, Makoto ; Masaki, Yoshinori ; Uenoyama, Maki ; Wakisaka, Hitoshi ; Ashida, Hiroshi ; Obara, Minoru. / In vitro gene transfer by the use of laser-induced stress wave : Effect of laser irradiation conditions on transfection efficiency. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2005 ; Vol. 5695. pp. 225-229.
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