Abstract
The aim is to evaluate if a difference in activity of monocytic cells can be described on superparamagnetic iron oxide (SPIO)-magnetic resonance imaging (MRI) in vitro when a low concentration of SPIO is administered. Human monocytic cells were stimulated with phorbol 12-myristate 13-acetate (PMA) or left non-stimulated for 24 h and then treated with SPIO with a final concentration of 7.2 μg Fe/ml for 48 h. They were collected, and an MRI phantom was prepared by suspending them in 4% gelatin and scanned using gradient-echo T2*-weighted and spin-echo T2-weighted imaging with two different echo times. The signal intensity and T2*/T2 relaxation times of each layer were also assessed. The number and degree of intracellular filling of cells positively stained with Berlin blue were evaluated microscopically. In addition, the heme oxygenase-1 expression in non-stimulated monocytic cells or monocytic cells stimulated with PMA was evaluated using semiquantitative reverse transcription and polymerase chain reaction. The signal reduction of cells stimulated with PMA was 3.9- to 5.0-fold and 2.7- to 3.1-fold greater than that of non-stimulated cells on the T2*- and T2-weighted images, respectively. These signal reductions were also visually confirmed. Estimated T2*/T2 relaxation times of cells stimulated with PMA and non-stimulated cells were 31.3/137.8 and 122.8/182.6 ms, respectively. Cells positively stained with Berlin blue were observed in 4.5% and 25.3% of non-stimulated cells and those stimulated with PMA, respectively (p < 0.05). The intracellular filling in cells stimulated with PMA was significantly larger than that in non-stimulated cells (p < 0.05). The expression of the heme oxygenase-1 gene in monocytic cells stimulated with PMA was 2.9-fold greater than that in non-stimulated monocytic cells. SPIO-MRI can visually describe a difference in activity of monocytic cells in vitro when a low concentration of SPIO is administered.
| Original language | English |
|---|---|
| Pages (from-to) | 638-642 |
| Number of pages | 5 |
| Journal | Computerized Medical Imaging and Graphics |
| Volume | 31 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Dec 1 2007 |
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All Science Journal Classification (ASJC) codes
- Radiological and Ultrasound Technology
- Radiology Nuclear Medicine and imaging
- Computer Vision and Pattern Recognition
- Health Informatics
- Computer Graphics and Computer-Aided Design
Cite this
In vitro imaging of human monocytic cellular activity using superparamagnetic iron oxide. / Nishie, Akihiro; Yoshimitsu, Kengo; Nakayama, Tomohiro; Hatakenaka, Masamitsu; Irie, Hiroyuki; Shioyama, Yoshiyuki; Matsuura, Shuji; Nishihara, Yunosuke; Kobayashi, Koji; Honda, Hiroshi.
In: Computerized Medical Imaging and Graphics, Vol. 31, No. 8, 01.12.2007, p. 638-642.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro imaging of human monocytic cellular activity using superparamagnetic iron oxide
AU - Nishie, Akihiro
AU - Yoshimitsu, Kengo
AU - Nakayama, Tomohiro
AU - Hatakenaka, Masamitsu
AU - Irie, Hiroyuki
AU - Shioyama, Yoshiyuki
AU - Matsuura, Shuji
AU - Nishihara, Yunosuke
AU - Kobayashi, Koji
AU - Honda, Hiroshi
PY - 2007/12/1
Y1 - 2007/12/1
N2 - The aim is to evaluate if a difference in activity of monocytic cells can be described on superparamagnetic iron oxide (SPIO)-magnetic resonance imaging (MRI) in vitro when a low concentration of SPIO is administered. Human monocytic cells were stimulated with phorbol 12-myristate 13-acetate (PMA) or left non-stimulated for 24 h and then treated with SPIO with a final concentration of 7.2 μg Fe/ml for 48 h. They were collected, and an MRI phantom was prepared by suspending them in 4% gelatin and scanned using gradient-echo T2*-weighted and spin-echo T2-weighted imaging with two different echo times. The signal intensity and T2*/T2 relaxation times of each layer were also assessed. The number and degree of intracellular filling of cells positively stained with Berlin blue were evaluated microscopically. In addition, the heme oxygenase-1 expression in non-stimulated monocytic cells or monocytic cells stimulated with PMA was evaluated using semiquantitative reverse transcription and polymerase chain reaction. The signal reduction of cells stimulated with PMA was 3.9- to 5.0-fold and 2.7- to 3.1-fold greater than that of non-stimulated cells on the T2*- and T2-weighted images, respectively. These signal reductions were also visually confirmed. Estimated T2*/T2 relaxation times of cells stimulated with PMA and non-stimulated cells were 31.3/137.8 and 122.8/182.6 ms, respectively. Cells positively stained with Berlin blue were observed in 4.5% and 25.3% of non-stimulated cells and those stimulated with PMA, respectively (p < 0.05). The intracellular filling in cells stimulated with PMA was significantly larger than that in non-stimulated cells (p < 0.05). The expression of the heme oxygenase-1 gene in monocytic cells stimulated with PMA was 2.9-fold greater than that in non-stimulated monocytic cells. SPIO-MRI can visually describe a difference in activity of monocytic cells in vitro when a low concentration of SPIO is administered.
AB - The aim is to evaluate if a difference in activity of monocytic cells can be described on superparamagnetic iron oxide (SPIO)-magnetic resonance imaging (MRI) in vitro when a low concentration of SPIO is administered. Human monocytic cells were stimulated with phorbol 12-myristate 13-acetate (PMA) or left non-stimulated for 24 h and then treated with SPIO with a final concentration of 7.2 μg Fe/ml for 48 h. They were collected, and an MRI phantom was prepared by suspending them in 4% gelatin and scanned using gradient-echo T2*-weighted and spin-echo T2-weighted imaging with two different echo times. The signal intensity and T2*/T2 relaxation times of each layer were also assessed. The number and degree of intracellular filling of cells positively stained with Berlin blue were evaluated microscopically. In addition, the heme oxygenase-1 expression in non-stimulated monocytic cells or monocytic cells stimulated with PMA was evaluated using semiquantitative reverse transcription and polymerase chain reaction. The signal reduction of cells stimulated with PMA was 3.9- to 5.0-fold and 2.7- to 3.1-fold greater than that of non-stimulated cells on the T2*- and T2-weighted images, respectively. These signal reductions were also visually confirmed. Estimated T2*/T2 relaxation times of cells stimulated with PMA and non-stimulated cells were 31.3/137.8 and 122.8/182.6 ms, respectively. Cells positively stained with Berlin blue were observed in 4.5% and 25.3% of non-stimulated cells and those stimulated with PMA, respectively (p < 0.05). The intracellular filling in cells stimulated with PMA was significantly larger than that in non-stimulated cells (p < 0.05). The expression of the heme oxygenase-1 gene in monocytic cells stimulated with PMA was 2.9-fold greater than that in non-stimulated monocytic cells. SPIO-MRI can visually describe a difference in activity of monocytic cells in vitro when a low concentration of SPIO is administered.
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UR - http://www.scopus.com/inward/citedby.url?scp=35348938431&partnerID=8YFLogxK
U2 - 10.1016/j.compmedimag.2007.07.004
DO - 10.1016/j.compmedimag.2007.07.004
M3 - Article
C2 - 17889504
AN - SCOPUS:35348938431
VL - 31
SP - 638
EP - 642
JO - Computerized Medical Imaging and Graphics
JF - Computerized Medical Imaging and Graphics
SN - 0895-6111
IS - 8
ER -