In vitro immunization can elicit the expansion of diverse repertoire of B cells from peripheral blood mononuclear cells

Shin Ei Matsumoto, Makiko Yamashita, Yoshinori Katakura, Eri Noguchi, Yoshihiro Aiba, Akira Ichikawa, Kiichiro Teruya, Sanetaka Shirahata

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We previously developed an in vitro immunization (IVI) protocol of human peripheral blood mononuclear cells (PBMC) for generating antigen-specific human antibodies. In order to clarify whether IVI protocolinduces antigen-specific B cell responses in PBMC, we analyzed family gene usage and sequence of the variable region gene of immunoglobulin heavy chain (VH gene) of the antibody produced from the in vitro immunized PBMC. Sequence homology analyses of VH gene demonstrated that a larger repertoire of B cells can be sensitized with mite-extract than with cholera toxin B subunit and rice allergen. Further, antigen-specific B cells were efficiently expanded by using CpG oligodeoxynucleotide as adjuvant. These results suggest that appropriate combination of sensitizing antigen and adjuvant is primarily important for expansion of antigen-specific B cells in IVI protocol.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalCytotechnology
Volume52
Issue number3
DOIs
Publication statusPublished - Nov 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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