In vitro induction of specific CD8 + T lymphocytes by tumor-associated antigenic peptides in patients with oral squamous cell carcinoma

Takeshi Toyoshima, Wataru Kumamaru, Jun nosuke Hayashida, Masahumi Moriyama, Ryoji Kitamura, Hideaki Tanaka, Akira Yamada, Kyogo Itoh, Seiji Nakamura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to clarify candidate peptides for peptide-based specific immunotherapy of patients with oral squamous cell carcinoma (SCC). Thirteen peptides were examined for in vitro induction of peptide-specific CD8 + T lymphocyte (CD8 +TL) activity in peripheral blood mononuclear cells from 35 patients with oral SCC. A correlation between the induction ability of CD8 +TL and in vivo immune response of host was carried out immunohistochemically in 23 patients. Peptide-specific activities of CD8 +TL for at least one peptide were detectable in 21/35 patients (60.0%). The potent peptides were SART-1 690 in 9/35 (25.7%), SART-2 93, and ART4 75 in 7/35 (20.0%), respectively. In the 9 patients with SART-1 690-specific activity, the whole of activities was significantly inducible for more number of other peptides compared to that in 26 patients without the activity (P=0.035). Cellular responses in 7 patients with SART-1 690-specific activity were significantly stronger than those in 16 patients without the activity (P=0.027). Furthermore, the number of CD3 + T cells around the SCC was also significantly different between the 2 groups of patients (P=0.041). In conclusion, SART-1 690, SART-2 93, and ART4 75 could be applicable as peptide-based specific immunotherapies for the majority of patients with oral SCC.

Original languageEnglish
Pages (from-to)86-91
Number of pages6
JournalCancer Letters
Volume322
Issue number1
DOIs
Publication statusPublished - Sep 1 2012

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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