In vitro interaction between tetracalcium phosphate-based cement and calvarial osteogenic cells

Y. Yoshimine, M. Sumi, R. Isobe, H. Anan, K. Maeda

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Newly-developed tetracalcium phosphate-based cement (4CP cement) and cells derived from neonatal rat calvaria were cocultured to study the in vitro reaction of osteoblastic cells to the biomaterial at light and electron microscopic levels. Three-dimensional nodular structures covered with active osteoblastic cells were formed in the periphery of the test material and they contained a mineralized tissue that exhibited features closely resembling bone formed in vivo. Ultrastructurally, the test material was circumscribed with an electron-dense structure, and was immediately adjacent to elongated cyoplasmic processes with intact morphology or collagen fibrils with periodic structures. Furthermore, the mineralization of the extracellular collagenous matrix occurred directly on the surface of the material. These in vitro findings suggest the ability of 4CP cement to bind directly with newly-formed hard tissues.

Original languageEnglish
Pages (from-to)2241-2245
Number of pages5
JournalBiomaterials
Volume17
Issue number23
DOIs
Publication statusPublished - Jan 1 1996

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Fingerprint Dive into the research topics of 'In vitro interaction between tetracalcium phosphate-based cement and calvarial osteogenic cells'. Together they form a unique fingerprint.

  • Cite this