In vitro measurement of post-natal changes in proliferating satellite cell frequency during rat muscle growth

Takahiro Suzuki, Hideyuki Takaishi, Tomowa Sakata, Mai Khoi Q. Do, Minako Hara, Akiko Sato, Wataru Mizunoya, Takanori Nishimura, Akihito Hattori, Yoshihide Ikeuchi, Ryuichi Tatsumi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Satellite cells, resident myogenic stem cells found in postnatal skeletal muscle, are most abundant during early postnatal development and sharply decline in frequency thereafter to adult levels in mice and rats. Therefore, postnatal changes in satellite cell mitotic activities are important aspects for further understanding a muscle growth strategy. In large meat-production animals, however, the traditional in vivo proliferation assay may be less realistic because it requires intra-peritoneal (ip) injection of huge dosage of mutagenic nucleosides, 3H-labeled thymidine or bromodeoxyuridine (BrdU), at each age-time of sacrifice. We report in the present pilot study using rats that in vivo proliferation activity of satellite cells can be evaluated by an in vitro BrdU-incorporation assay in early cultures. Briefly, satellite cells were prepared from upper hind-limb and back muscles and maintained for 24 h with imposing by BrdU addition for the last 2 h, followed by the regular immunocytochemistry for determining BrdU-incorporated cell percentage. This in vitro assay demonstrated a rapid decrease in proliferating satellite cell frequency to the adult level during about 3-month period after birth, and yielded a high correlation to the measurements by the in vivo BrdU ip-injection method during the postnatal period examined from day-2 to month-11. The in vitro proliferation assay may be further adaptable for large domestic animals by the combination with a muscle biopsy technique that enables age-interval sampling from the same growing animals.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalAnimal Science Journal
Volume81
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

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Bromodeoxyuridine
Muscles
muscles
rats
Growth
assays
cells
intraperitoneal injection
Skeletal Muscle Satellite Cells
Back Muscles
Injections
Domestic Animals
Nucleosides
Upper Extremity
postnatal development
nucleosides
Thymidine
Meat
meat production
postpartum period

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

In vitro measurement of post-natal changes in proliferating satellite cell frequency during rat muscle growth. / Suzuki, Takahiro; Takaishi, Hideyuki; Sakata, Tomowa; Do, Mai Khoi Q.; Hara, Minako; Sato, Akiko; Mizunoya, Wataru; Nishimura, Takanori; Hattori, Akihito; Ikeuchi, Yoshihide; Tatsumi, Ryuichi.

In: Animal Science Journal, Vol. 81, No. 2, 01.04.2010, p. 245-251.

Research output: Contribution to journalArticle

Suzuki, T, Takaishi, H, Sakata, T, Do, MKQ, Hara, M, Sato, A, Mizunoya, W, Nishimura, T, Hattori, A, Ikeuchi, Y & Tatsumi, R 2010, 'In vitro measurement of post-natal changes in proliferating satellite cell frequency during rat muscle growth', Animal Science Journal, vol. 81, no. 2, pp. 245-251. https://doi.org/10.1111/j.1740-0929.2009.00734.x
Suzuki, Takahiro ; Takaishi, Hideyuki ; Sakata, Tomowa ; Do, Mai Khoi Q. ; Hara, Minako ; Sato, Akiko ; Mizunoya, Wataru ; Nishimura, Takanori ; Hattori, Akihito ; Ikeuchi, Yoshihide ; Tatsumi, Ryuichi. / In vitro measurement of post-natal changes in proliferating satellite cell frequency during rat muscle growth. In: Animal Science Journal. 2010 ; Vol. 81, No. 2. pp. 245-251.
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