In vitro neuroprotective activities of compounds from Angelica shikokiana Makino

Amira Mira, Shuntaro Yamashita, Yoshinori Katakura, Kuniyoshi Shimizu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Angelica shikokiana is widely marketed in Japan as a dietary food supplement. With a focus on neurodegenerative conditions such as Alzheimer's disease, the aerial part was extracted and through bio-guided fractionation, fifteen compounds [α-glutinol, β-amyrin, kaempferol, luteolin, quercetin, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, methyl chlorogenate, chlorogenic acid, hyuganin E, 5-(hydroxymethyl)-2-furaldehyde, β-sitosterol-3-O-glucoside, adenosine (isolated for the first time from A. shikokiana), isoepoxypteryxin and isopteryxin] were isolated. Isolated compounds were evaluated for in vitro neuroprotection using acetylcholine esterase inhibitory, protection against hydrogen peroxide and amyloid β peptide (Aβ25-35)-induced neurotoxicity in neuro-2A cells, scavenging of hydroxyl radicals and intracellular reactive oxygen species and thioflavin T assays. Quercetin showed the strongest AChE inhibition (IC50 value = 35.5 μM) through binding to His-440 and Tyr-70 residues at the catalytic and anionic sites of acetylcholine esterase, respectively. Chlorogenic acid, its methyl ester, quercetin and luteolin could significantly protect neuro-2A cells against H2O2-induced neurotoxicity and scavenge hydroxyl radical and intracellular reactive oxygen species. Kaempferol-3-O-rutinoiside, hyuganin E and isoepoxypteryxin significantly decreased Aβ25-35-induced neurotoxicity and Th-T fluorescence. To the best of our knowledge, this is the first report about neuroprotection of hyuganin E and isoepoxypteryxin against Aβ25-35-induced neurotoxicity.

Original languageEnglish
Pages (from-to)4813-4832
Number of pages20
JournalMolecules
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

Fingerprint

Angelica
acetylcholine
glucosides
Quercetin
hydroxyl radicals
Luteolin
Chlorogenic Acid
Esterases
Dietary Supplements
adenosines
Hydroxyl Radical
acids
Acetylcholine
Reactive Oxygen Species
scavenging
supplements
oxygen
cells
hydrogen peroxide
food

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

In vitro neuroprotective activities of compounds from Angelica shikokiana Makino. / Mira, Amira; Yamashita, Shuntaro; Katakura, Yoshinori; Shimizu, Kuniyoshi.

In: Molecules, Vol. 20, No. 3, 01.03.2015, p. 4813-4832.

Research output: Contribution to journalArticle

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