In vitro PMP import analysis using cell-free synthesized PMP and isolated peroxisomes

Yuqiong Liu; Masanori Honsho; Yukio Fujiki

doi:10.1007/978-1-4939-6937-1_19

In vitro PMP import analysis using cell-free synthesized PMP and isolated peroxisomes

Yuqiong Liu, Masanori Honsho, Yukio Fujiki

Faculty of Medical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The Pex19p-and Pex3p-dependent direct import of peroxisomal membrane proteins (PMPs), termed the class I pathway, can be reconstituted in vitro by incubating cell-free synthesized PMPs with highly purified peroxisomes at 26 °C for 1 h. This method ensures that the proteins targeted to peroxisomes are imported directly without involvement of other organelles.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	207-212
Number of pages	6
DOIs	https://doi.org/10.1007/978-1-4939-6937-1_19
Publication status	Published - Jan 1 2017

Publication series

Name	Methods in Molecular Biology
Volume	1595
ISSN (Print)	1064-3745

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-6937-1_19

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abstract = "The Pex19p-and Pex3p-dependent direct import of peroxisomal membrane proteins (PMPs), termed the class I pathway, can be reconstituted in vitro by incubating cell-free synthesized PMPs with highly purified peroxisomes at 26 °C for 1 h. This method ensures that the proteins targeted to peroxisomes are imported directly without involvement of other organelles.",

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