@inbook{be3665d7403d40ef9ea36e3ee84531e6,
title = "In vitro PMP import analysis using cell-free synthesized PMP and isolated peroxisomes",
abstract = "The Pex19p-and Pex3p-dependent direct import of peroxisomal membrane proteins (PMPs), termed the class I pathway, can be reconstituted in vitro by incubating cell-free synthesized PMPs with highly purified peroxisomes at 26 °C for 1 h. This method ensures that the proteins targeted to peroxisomes are imported directly without involvement of other organelles.",
author = "Yuqiong Liu and Masanori Honsho and Yukio Fujiki",
year = "2017",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-6937-1_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "207--212",
booktitle = "Methods in Molecular Biology",
}