In vitro production of functionally mature hepatocytes from prospectively isolated hepatic stem cells

Atsushi Suzuki, Hiromitsu Nakauchi, Hideki Taniguchi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hepatocyte transplantation and artificial organ hepatic support require a number of functionally mature hepatocytes. However, their growth activity and functional behaviors are much smaller in culture after isolation from the liver. We examined whether continuously differentiating hepatocytes from multipotent hepatic stem cells that were isolated by using flow cytometry and propagated clonally in culture could be a source of clinical application. They actually gave rise to cells that were functionally equal to mature hepatocytes found in the adult liver, which secreted albumin into culture medium and metabolized harmful ammonium into urea. These data suggest that stem cell-derived hepatocytes are a useful cell source for developing therapeutic strategies, such as cell transplantation, gene therapy, and artificial liver organ to treat various liver disorders.

Original languageEnglish
Pages (from-to)469-473
Number of pages5
JournalCell Transplantation
Volume12
Issue number5
DOIs
Publication statusPublished - 2003

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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