In vitro selection of RNA aptamers for a small-molecule dye

Asako Murata, Shin Ichi Sato

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Artificial riboswitches can be generated by functional coupling of an RNA aptamer for synthetic small molecule to an expression platform. RNA aptamers that can bind strongly and selectively to their target are feasible to be used for obtaining more potent artificial riboswitches. In this chapter, we describe tips and notes for in vitro selection of RNA aptamers targeting synthetic small molecules.

Original languageEnglish
Title of host publicationArtificial Riboswitches
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages17-28
Number of pages12
ISBN (Print)9781627037549
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1111
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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