in vitro studies of human choroidal endothelial cells

Taiji Sakamoto, Hitomi Sakamoto, David R. Hinton, Christine Spee, Tatsuro Ishibashi, Stephen J. Ryan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Vascular endothelial cells play an important role in progression or healing of various retinal or choroidal diseases and they have a broad organ specificity. We have grown and studied human choroidal endothelial cells (CECs) cultured in a collagen gel, where they form tube-like structures. CECs were differentially isolated from choroidal tissues and cultured to near homogeneity. The cells were embedded in a type I collagen gel in 24-well culture dishes and incubated with M199 medium containing 10% fetal calf serum. Gels were observed using phase contrast microscopy and were evaluated histologically by light and electron microscopy. The CECs were strongly positive for factor VIH-related antigen and actively ingested diI-acetylated LDL, indicating their endothelial nature. Ultrastructural analysis of the tube-like structures revealed a central lumen surrounded by cells joined apically by junctions and showing prominent pinocytotic activity, fenestrations and basement membrane formation; these features are typical of CECs in vivo. This preparation should be a useful tool to study the development of physiology and pathology of choroidal endothelium.

Original languageEnglish
Pages (from-to)621-627
Number of pages7
JournalCurrent Eye Research
Volume14
Issue number8
DOIs
Publication statusPublished - Jan 1 1995

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Endothelial Cells
Gels
Choroid Diseases
Phase-Contrast Microscopy
Retinal Diseases
Organ Specificity
Collagen Type I
Basement Membrane
Endothelium
Electron Microscopy
Collagen
In Vitro Techniques
Pathology
Light
Antigens
Serum

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Sakamoto, T., Sakamoto, H., Hinton, D. R., Spee, C., Ishibashi, T., & Ryan, S. J. (1995). in vitro studies of human choroidal endothelial cells. Current Eye Research, 14(8), 621-627. https://doi.org/10.3109/02713689508998488

in vitro studies of human choroidal endothelial cells. / Sakamoto, Taiji; Sakamoto, Hitomi; Hinton, David R.; Spee, Christine; Ishibashi, Tatsuro; Ryan, Stephen J.

In: Current Eye Research, Vol. 14, No. 8, 01.01.1995, p. 621-627.

Research output: Contribution to journalArticle

Sakamoto, T, Sakamoto, H, Hinton, DR, Spee, C, Ishibashi, T & Ryan, SJ 1995, 'in vitro studies of human choroidal endothelial cells', Current Eye Research, vol. 14, no. 8, pp. 621-627. https://doi.org/10.3109/02713689508998488
Sakamoto, Taiji ; Sakamoto, Hitomi ; Hinton, David R. ; Spee, Christine ; Ishibashi, Tatsuro ; Ryan, Stephen J. / in vitro studies of human choroidal endothelial cells. In: Current Eye Research. 1995 ; Vol. 14, No. 8. pp. 621-627.
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