In vitro succinate dehydrogenase chemosensitivity of gastric carcinoma-relationship to DNA content

A. Saito, D. Korenaga, Y. Maehara, H. Baba, T. Okamura, K. Sugimachi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Relationships between in vitro chemosensitivity and cell nuclear DNA content were investigated in malignant cells from 41 patients exhibiting advanced gastric carcinoma. The chemosensitivity was evaluated by measuring the succinate dehydrogenase (SD) activity in drug-exposed cancer cells and the DNA content was microspectrophotometrically determined. Following exposure of malignant tissue to carboquone (CQ) and cisplatin (DDP), the mean SD activity in cells displaying a relatively regular DNA distribution (type II) was significantly higher than that in those exhibiting a widely scattered DNA distribution (type IV;P<0.01 in CQ, P<0.05 in DDP). A similar tendency was recognized in cells that were treated with aclacinomycin A (ACR), Adriamycin (ADM), and mitomycin C (MMC). Such a decrease in SD activity in cells exhibiting a type IV pattern was remarkable, especially in cases undifferentiated adenocarcinoma. Mitotic counting analysis revealed a significantly higher value for DNA pattern type IV as compared with the findings for type II (P<0.01). These results demonstrate that gastric carcinoma displaying a high malignant potentiality shows a better response to antitumor drugs. Adjuvant chemotherapy prescribed following drug-sensitivity testing should be effective against such tumors.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalCancer chemotherapy and pharmacology
Volume29
Issue number3
DOIs
Publication statusPublished - May 1992

All Science Journal Classification (ASJC) codes

  • Oncology
  • Toxicology
  • Pharmacology
  • Cancer Research
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'In vitro succinate dehydrogenase chemosensitivity of gastric carcinoma-relationship to DNA content'. Together they form a unique fingerprint.

Cite this