In vivo and in vitro complementation study comparing the function of DnaK chaperone systems from halophilic lactic acid bacterium Tetragenococcus halophilus and Escherichia coli

Shinya Sugimoto, Kozue Saruwatari, Chihana Higashi, Keigo Tsuruno, Shunsuke Matsumoto, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

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Abstract

In this study, we characterized the DnaK chaperone system from Tetragenococcus halophilus, a halophilic lactic acid bacterium. An in vivo complementation test showed that under heat stress conditions, T. halophilus DnaK did not rescue the growth of the Escherichia coli dnaK deletion mutant, whereas T. halophilus DnaJ and GrpE complemented the corresponding mutations of E. coli. Purified T. halophilus DnaK showed intrinsic weak ATPase activity and holding chaperone activity in vitro, but T. halophilus DnaK did not cooperate with the purified DnaJ and GrpE from either T. halophilus or E. coli in ATP hydrolysis or luciferase-refolding reactions under the conditions tested. E. coli DnaK, however, cross-reacted with those from both bacteria. This difference in the cooperation with DnaJ and GrpE appears to result in an inability of T. halophilus DnaK to replace the in vivo function of the DnaK chaperone of E. coli.

Original languageEnglish
Pages (from-to)811-822
Number of pages12
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number3
DOIs
Publication statusPublished - Apr 7 2008

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Enterococcaceae
Tetragenococcus halophilus
Lactic acid
Escherichia coli
lactic acid bacteria
Lactic Acid
Bacteria
Genetic Complementation Test
Adenosinetriphosphate
Luciferases
Adenosine Triphosphatases
Hydrolysis
Hot Temperature
Adenosine Triphosphate
luciferase
In Vitro Techniques
adenosinetriphosphatase
heat stress
Mutation
hydrolysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

In vivo and in vitro complementation study comparing the function of DnaK chaperone systems from halophilic lactic acid bacterium Tetragenococcus halophilus and Escherichia coli. / Sugimoto, Shinya; Saruwatari, Kozue; Higashi, Chihana; Tsuruno, Keigo; Matsumoto, Shunsuke; Nakayama, Jiro; Sonomoto, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 3, 07.04.2008, p. 811-822.

Research output: Contribution to journalArticle

Sugimoto, Shinya ; Saruwatari, Kozue ; Higashi, Chihana ; Tsuruno, Keigo ; Matsumoto, Shunsuke ; Nakayama, Jiro ; Sonomoto, Kenji. / In vivo and in vitro complementation study comparing the function of DnaK chaperone systems from halophilic lactic acid bacterium Tetragenococcus halophilus and Escherichia coli. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 3. pp. 811-822.
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