In vivo blockade of T cell development reveals alternative pathways for generation of intraepithelial lymphocytes in mice

Surenchimeg Mondoon, Kensuke Shibata, Yasunobu Yoshikai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Intraepithelial lymphocytes (IELs) are resident cells localized within the intestinal epithelia and play an important role in regulating gut inflammations and host defense against pathogens. CD8α+ TCRαβ+ IELs are heterogeneous populations that are generated from T cell precursors including CD4 CD8α double-negative (DN) cells and CD4+ CD8α+ double-positive (DP) cells. However, developmental pathways of TCRαβ+ IELs remained unclear. To gain insight into the mechanisms, we generated mice (Bcl11bΔDN2 mice) that lack thymic precursors (DN CD5+ TCRβ+ cells) for CD4 CD8αα+ TCRαβ+ IELs. Unexpectedly, we found that, in the absence of the precursors in thymi of Bcl11bΔDN2 mice, CD4 CD8αα+ TCRαβ+ IELs were still present in the intestine though the number was reduced. Adoptive transfer experiment showed that their precursors were highly enriched in CD8α+ TCRβ thymocytes. The CD4 CD8αα+ TCRαβ+ IELs in Bcl11bΔDN2 mice are distinguished by Thy1.2 expression and are indeed present in WT mice. Taken together, our study reveal a novel developmental pathway for CD8αα+ TCRαβ+ IELs.

Original languageEnglish
Pages (from-to)40-46
Number of pages7
JournalImmunology Letters
Volume191
DOIs
Publication statusPublished - Nov 1 2017

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Lymphocytes
T-Lymphocytes
T-Lymphoid Precursor Cells
Adoptive Transfer
Thymocytes
Intestinal Mucosa
Thymus Gland
Intestines
Inflammation
Population

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

In vivo blockade of T cell development reveals alternative pathways for generation of intraepithelial lymphocytes in mice. / Mondoon, Surenchimeg; Shibata, Kensuke; Yoshikai, Yasunobu.

In: Immunology Letters, Vol. 191, 01.11.2017, p. 40-46.

Research output: Contribution to journalArticle

Mondoon, Surenchimeg ; Shibata, Kensuke ; Yoshikai, Yasunobu. / In vivo blockade of T cell development reveals alternative pathways for generation of intraepithelial lymphocytes in mice. In: Immunology Letters. 2017 ; Vol. 191. pp. 40-46.
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