In Vivo Curdlan/Cellulose Bionanocomposite Synthesis by Genetically Modified Gluconacetobacter xylinus

Ju Fang, Shin Kawano, Kenji Tajima, Tetsuo Kondo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Bacterial cellulose pellicle produced by Gluconacetobacter xylinus (G. xylinus) is one of the best biobased materials having a unique supernetwork structure with remarkable physiochemical properties for a wide range of medical and tissue-engineering applications. It is still necessary to modify them to obtain materials suitable for biomedical use with satisfactory mechanical strength, biodegradability, and bioactivity. The aim of this research was to develop a gene-transformation route for the production of bacterial cellulose/Curdlan (β-1,3-glucan) nanocomposites by separate but simultaneous in vivo synthesis of cellulose and Curdlan. Modification of the cellulose-nanofiber-producing system of G. xylinus enabled Curdlan to be synthesized simultaneously with cellulose nanofibers in vivo, resulting in biopreparation of nanocomposites. The obtained Curdlan/cellulose composites were characterized, and their properties were compared with those of normal bacterial cellulose pellicles, indicating that Curdlan mixed with the cellulose nanofibers at the nanoscale without disruption of the nanofiber network structure in the pellicle.

Original languageEnglish
Pages (from-to)3154-3160
Number of pages7
JournalBiomacromolecules
Volume16
Issue number10
DOIs
Publication statusPublished - Oct 12 2015

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Cellulose
Nanofibers
Nanocomposites
Glucans
curdlan
Biodegradability
Bioactivity
Tissue engineering
Strength of materials
Genes
Composite materials

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

In Vivo Curdlan/Cellulose Bionanocomposite Synthesis by Genetically Modified Gluconacetobacter xylinus. / Fang, Ju; Kawano, Shin; Tajima, Kenji; Kondo, Tetsuo.

In: Biomacromolecules, Vol. 16, No. 10, 12.10.2015, p. 3154-3160.

Research output: Contribution to journalArticle

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