Abstract
We developed a porous chitosan/ hydroxyapatite (HAp) composite, in which the HAp nanocrystals align along the chitosan molecules, and examined the biocompatibility, osteoinductive activity, and the ability to act as a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) of this novel biomaterial. The composite was subcutaneously implanted into the backs of 11-week-old SD rats, with or without rhBMP-2 (5 μg). At 2 and 8 weeks after the implantation, the composite was explanted for morphohistological evaluation. In the presence of rhBMP-2, ectopic bone formation was found at 2 weeks and maturation of the newly formed bone around the composite at 8 weeks. Chitosan/HAp composite alone caused little inflammation, and new blood vessel growth and multinucleated giant cells were found around the composite, accompanied with roughening of the surface due to degradation at 2 weeks; however, neither cartilage nor bone formation was found around the composite. With rhBMP-2, the bioabsorption of the composite was accelerated as the rhBMP-2-induced bone matured. Histomorphometrical analysis showed that the mean value of the composite areas with rhBMP-2 was significantly smaller than that without rhBMT-2 at 2 and 8 weeks after the implantation. These results suggested that the novel chitosan/ HAp composite was an effective bioabsorbable material as a carrier of rhBMP-2.
| Original language | English |
|---|---|
| Pages (from-to) | 181-186 |
| Number of pages | 6 |
| Journal | Journal of Hard Tissue Biology |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Dec 1 2010 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Biochemistry
- Biomaterials
- Orthopedics and Sports Medicine
- Dentistry(all)
- Cell Biology
Cite this
In vivo evaluation of a novel chitosan/HAP composite biomaterial as a carrier of rhBMP-2. / Kashiwazaki, Haruhiko; Yamaguchi, Keisuke; Harada, Naoki; Akazawa, Toshiyuki; Murata, Masaru; Iizuka, Tadashi; Ikoma, Toshiyuki; Tanaka, Junzo; Inoue, Nobuo.
In: Journal of Hard Tissue Biology, Vol. 19, No. 3, 01.12.2010, p. 181-186.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo evaluation of a novel chitosan/HAP composite biomaterial as a carrier of rhBMP-2
AU - Kashiwazaki, Haruhiko
AU - Yamaguchi, Keisuke
AU - Harada, Naoki
AU - Akazawa, Toshiyuki
AU - Murata, Masaru
AU - Iizuka, Tadashi
AU - Ikoma, Toshiyuki
AU - Tanaka, Junzo
AU - Inoue, Nobuo
PY - 2010/12/1
Y1 - 2010/12/1
N2 - We developed a porous chitosan/ hydroxyapatite (HAp) composite, in which the HAp nanocrystals align along the chitosan molecules, and examined the biocompatibility, osteoinductive activity, and the ability to act as a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) of this novel biomaterial. The composite was subcutaneously implanted into the backs of 11-week-old SD rats, with or without rhBMP-2 (5 μg). At 2 and 8 weeks after the implantation, the composite was explanted for morphohistological evaluation. In the presence of rhBMP-2, ectopic bone formation was found at 2 weeks and maturation of the newly formed bone around the composite at 8 weeks. Chitosan/HAp composite alone caused little inflammation, and new blood vessel growth and multinucleated giant cells were found around the composite, accompanied with roughening of the surface due to degradation at 2 weeks; however, neither cartilage nor bone formation was found around the composite. With rhBMP-2, the bioabsorption of the composite was accelerated as the rhBMP-2-induced bone matured. Histomorphometrical analysis showed that the mean value of the composite areas with rhBMP-2 was significantly smaller than that without rhBMT-2 at 2 and 8 weeks after the implantation. These results suggested that the novel chitosan/ HAp composite was an effective bioabsorbable material as a carrier of rhBMP-2.
AB - We developed a porous chitosan/ hydroxyapatite (HAp) composite, in which the HAp nanocrystals align along the chitosan molecules, and examined the biocompatibility, osteoinductive activity, and the ability to act as a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) of this novel biomaterial. The composite was subcutaneously implanted into the backs of 11-week-old SD rats, with or without rhBMP-2 (5 μg). At 2 and 8 weeks after the implantation, the composite was explanted for morphohistological evaluation. In the presence of rhBMP-2, ectopic bone formation was found at 2 weeks and maturation of the newly formed bone around the composite at 8 weeks. Chitosan/HAp composite alone caused little inflammation, and new blood vessel growth and multinucleated giant cells were found around the composite, accompanied with roughening of the surface due to degradation at 2 weeks; however, neither cartilage nor bone formation was found around the composite. With rhBMP-2, the bioabsorption of the composite was accelerated as the rhBMP-2-induced bone matured. Histomorphometrical analysis showed that the mean value of the composite areas with rhBMP-2 was significantly smaller than that without rhBMT-2 at 2 and 8 weeks after the implantation. These results suggested that the novel chitosan/ HAp composite was an effective bioabsorbable material as a carrier of rhBMP-2.
UR - http://www.scopus.com/inward/record.url?scp=79751521764&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79751521764&partnerID=8YFLogxK
U2 - 10.2485/jhtb.19.181
DO - 10.2485/jhtb.19.181
M3 - Article
AN - SCOPUS:79751521764
VL - 19
SP - 181
EP - 186
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
SN - 1341-7649
IS - 3
ER -