In vivo evaluation of a novel chitosan/HAP composite biomaterial as a carrier of rhBMP-2

Haruhiko Kashiwazaki, Keisuke Yamaguchi, Naoki Harada, Toshiyuki Akazawa, Masaru Murata, Tadashi Iizuka, Toshiyuki Ikoma, Junzo Tanaka, Nobuo Inoue

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed a porous chitosan/ hydroxyapatite (HAp) composite, in which the HAp nanocrystals align along the chitosan molecules, and examined the biocompatibility, osteoinductive activity, and the ability to act as a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) of this novel biomaterial. The composite was subcutaneously implanted into the backs of 11-week-old SD rats, with or without rhBMP-2 (5 μg). At 2 and 8 weeks after the implantation, the composite was explanted for morphohistological evaluation. In the presence of rhBMP-2, ectopic bone formation was found at 2 weeks and maturation of the newly formed bone around the composite at 8 weeks. Chitosan/HAp composite alone caused little inflammation, and new blood vessel growth and multinucleated giant cells were found around the composite, accompanied with roughening of the surface due to degradation at 2 weeks; however, neither cartilage nor bone formation was found around the composite. With rhBMP-2, the bioabsorption of the composite was accelerated as the rhBMP-2-induced bone matured. Histomorphometrical analysis showed that the mean value of the composite areas with rhBMP-2 was significantly smaller than that without rhBMT-2 at 2 and 8 weeks after the implantation. These results suggested that the novel chitosan/ HAp composite was an effective bioabsorbable material as a carrier of rhBMP-2.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalJournal of Hard Tissue Biology
Volume19
Issue number3
DOIs
Publication statusPublished - Dec 1 2010
Externally publishedYes

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Chitosan
Biocompatible Materials
Biomaterials
Bone
Proteins
Durapatite
Composite materials
Hydroxyapatite
Osteogenesis
Bone and Bones
Giant Cells
recombinant human bone morphogenetic protein-2
Nanoparticles
Cartilage
Blood Vessels
Blood vessels
Biocompatibility
Inflammation
Nanocrystals
Rats

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

Cite this

In vivo evaluation of a novel chitosan/HAP composite biomaterial as a carrier of rhBMP-2. / Kashiwazaki, Haruhiko; Yamaguchi, Keisuke; Harada, Naoki; Akazawa, Toshiyuki; Murata, Masaru; Iizuka, Tadashi; Ikoma, Toshiyuki; Tanaka, Junzo; Inoue, Nobuo.

In: Journal of Hard Tissue Biology, Vol. 19, No. 3, 01.12.2010, p. 181-186.

Research output: Contribution to journalArticle

Kashiwazaki, H, Yamaguchi, K, Harada, N, Akazawa, T, Murata, M, Iizuka, T, Ikoma, T, Tanaka, J & Inoue, N 2010, 'In vivo evaluation of a novel chitosan/HAP composite biomaterial as a carrier of rhBMP-2', Journal of Hard Tissue Biology, vol. 19, no. 3, pp. 181-186. https://doi.org/10.2485/jhtb.19.181
Kashiwazaki, Haruhiko ; Yamaguchi, Keisuke ; Harada, Naoki ; Akazawa, Toshiyuki ; Murata, Masaru ; Iizuka, Tadashi ; Ikoma, Toshiyuki ; Tanaka, Junzo ; Inoue, Nobuo. / In vivo evaluation of a novel chitosan/HAP composite biomaterial as a carrier of rhBMP-2. In: Journal of Hard Tissue Biology. 2010 ; Vol. 19, No. 3. pp. 181-186.
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