In vivo gene transfer into muscle via electro-sonoporation

Yo Ichi Yamashita, Mitsuo Shimada, Katsuro Tachibana, Norifumi Harimoto, Eiji Tsujita, Ken Shirabe, Jun Ichi Miyazaki, Keizo Sugimachi

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Among the nonviral techniques for gene transfer in vivo, electroporation is simple, potent, inexpensive, and safe. To upregulate the expression levels of the transferred gene, we investigated the applicability of in vivo electro-sonoporation, which consists of a combination of electric pulse and ultrasound, for gene transfer using plasmid DNA encoding luciferase and mouse interleukin-12 (mIL-12). The quadriceps muscles of mice were injected with plasmid DNA, then sonoporated for 5 min, and electroporated by a pair of electrode plates at the middle of the duration of sonoporation. Three days later, mice that had undergone electro-sonoporation demonstrated twofold higher luciferase activity and low tissue damage in quadriceps muscle compared to mice having undergone electroporation alone. Serum mIL-12 levels in mice that had undergone electro-sonoporation (peaking at 25.5 ng/ml) were twofold higher after gene transfer than were those in mice having undergone electroporation alone (peaking at 14.3 ng/ml), and maintained high serum level of 13.9 ng/ml at 28 days after gene transfer. The efficacy of gene transfer via electro-sonoporation was superior when the plasmid DNA solution was 0.85% NaCI compared to albumin microbubble echo-contrast material. These results demonstrated that gene transfer into muscle via electro-sonoporation could provide a new potent nonviral technique for gene transfer in vivo.

Original languageEnglish
Pages (from-to)2079-2084
Number of pages6
JournalHuman Gene Therapy
Volume13
Issue number17
DOIs
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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  • Cite this

    Yamashita, Y. I., Shimada, M., Tachibana, K., Harimoto, N., Tsujita, E., Shirabe, K., Miyazaki, J. I., & Sugimachi, K. (2002). In vivo gene transfer into muscle via electro-sonoporation. Human Gene Therapy, 13(17), 2079-2084. https://doi.org/10.1089/10430340260395929