In Vivo Human Cartilage Formation in Three-Dimensional Bioprinted Constructs with a Novel Bacterial Nanocellulose Bioink

Peter Apelgren, Erdem Karabulut, Matteo Amoroso, Athanasios Mantas, Héctor Martínez Ávila, Lars Kölby, Tetsuo Kondo, Guillermo Toriz, Paul Gatenholm

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bacterial nanocellulose (BNC) is a 3D network of nanofibrils exhibiting excellent biocompatibility. Here, we present the aqueous counter collision (ACC) method of BNC disassembly to create bioink with suitable properties for cartilage-specific 3D-bioprinting. BNC was disentangled by ACC, and fibril characteristics were analyzed. Bioink printing fidelity and shear-thinning properties were evaluated. Cell-laden bioprinted grid constructs (5 × 5 × 1 mm3) containing human nasal chondrocytes (10 M mL-1) were implanted in nude mice and explanted after 30 and 60 days. Both ACC and hydrolysis resulted in significantly reduced fiber lengths, with ACC resulting in longer fibrils and fewer negative charges relative to hydrolysis. Moreover, ACC-BNC bioink showed outstanding printability, postprinting mechanical stability, and structural integrity. In vivo, cell-laden structures were rapidly integrated, maintained structural integrity, and showed chondrocyte proliferation, with 32.8 ± 13.8 cells per mm2 observed after 30 days and 85.6 ± 30.0 cells per mm2 at day 60 (p = 0.002). Furthermore, a full-thickness skin graft was attached and integrated completely on top of the 3D-bioprinted construct. The novel ACC disentanglement technique makes BNC biomaterial highly suitable for 3D-bioprinting and clinical translation, suggesting cell-laden 3D-bioprinted ACC-BNC as a promising solution for cartilage repair.

Original languageEnglish
Pages (from-to)2482-2490
Number of pages9
JournalACS Biomaterials Science and Engineering
Volume5
Issue number5
DOIs
Publication statusPublished - May 13 2019

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Cartilage
Structural integrity
Hydrolysis
Shear thinning
Mechanical stability
Biocompatible Materials
Biocompatibility
Biomaterials
Grafts
Printing
Skin
Repair
Fibers

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

In Vivo Human Cartilage Formation in Three-Dimensional Bioprinted Constructs with a Novel Bacterial Nanocellulose Bioink. / Apelgren, Peter; Karabulut, Erdem; Amoroso, Matteo; Mantas, Athanasios; Martínez Ávila, Héctor; Kölby, Lars; Kondo, Tetsuo; Toriz, Guillermo; Gatenholm, Paul.

In: ACS Biomaterials Science and Engineering, Vol. 5, No. 5, 13.05.2019, p. 2482-2490.

Research output: Contribution to journalArticle

Apelgren, P, Karabulut, E, Amoroso, M, Mantas, A, Martínez Ávila, H, Kölby, L, Kondo, T, Toriz, G & Gatenholm, P 2019, 'In Vivo Human Cartilage Formation in Three-Dimensional Bioprinted Constructs with a Novel Bacterial Nanocellulose Bioink', ACS Biomaterials Science and Engineering, vol. 5, no. 5, pp. 2482-2490. https://doi.org/10.1021/acsbiomaterials.9b00157
Apelgren, Peter ; Karabulut, Erdem ; Amoroso, Matteo ; Mantas, Athanasios ; Martínez Ávila, Héctor ; Kölby, Lars ; Kondo, Tetsuo ; Toriz, Guillermo ; Gatenholm, Paul. / In Vivo Human Cartilage Formation in Three-Dimensional Bioprinted Constructs with a Novel Bacterial Nanocellulose Bioink. In: ACS Biomaterials Science and Engineering. 2019 ; Vol. 5, No. 5. pp. 2482-2490.
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