In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis

Shinsuke Miyahara, Lama Almulki, Kousuke Noda, Toru Nakazawa, Toshio Hisatomi, Shintaro Nakao, Kennard L. Thomas, Alexander Schering, Souska Zandi, Sonja Frimmel, Faryan Tayyari, Rebecca C. Garland, Joan W. Miller, Evangelos S. Gragoudas, Sharmila Masli, Ali Hafezi-Moghadam

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Early detection of ocular inflammation may prevent the occurrence of structural damage or vision loss. Here, we introduce a novel noninvasive technique for molecular imaging and quantitative evaluation of endothelial injury in the choriocapillaris of live animals, which detects disease earlier than currently possible. Using an established model of ocular inflammation, endotoxin-induced uveitis (EIU), we visualized the rolling and adhesive interaction of fluorescent microspheres conjugated to recombinant P-selectin glycoprotein ligand-Ig (rPSGL-Ig) in choriocapillaris using a scanning laser ophthalmoscope (SLO). The number of rolling microspheres in the choriocapillaris peaked 4-10 h after LPS injection. The number of the accumulated microspheres peaked 4 h after LPS injection in the temporal choriocapillaris and 4 and 36 h after LPS injection in the central areas around the optic disk. Furthermore, we semiquantified the levels of P-selectin mRNA expression in the choroidal vessels by reverse transcription-PCR and found its pattern to match the functional microsphere interactions, with a peak at 4 h after LPS injection. These results indicate that PSGL-1-conjugated fluorescent microspheres allow specific detection of endothelial P-selectin expression in vivo and noninvasive assessment of endothelial injury. This technique may help to diagnose subclinical signs of ocular inflammatory diseases.

Original languageEnglish
Pages (from-to)1973-1980
Number of pages8
JournalFASEB Journal
Volume22
Issue number6
DOIs
Publication statusPublished - Jun 1 2008

Fingerprint

Uveitis
Microspheres
Endotoxins
P-Selectin
Imaging techniques
Wounds and Injuries
Injections
Inflammation
Ophthalmoscopes
Molecular imaging
Molecular Imaging
Eye Diseases
Optic Disk
P-Glycoprotein
Transcription
Adhesives
Reverse Transcription
Optics
Glycoproteins
Animals

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Miyahara, S., Almulki, L., Noda, K., Nakazawa, T., Hisatomi, T., Nakao, S., ... Hafezi-Moghadam, A. (2008). In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis. FASEB Journal, 22(6), 1973-1980. https://doi.org/10.1096/fj.07-096891

In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis. / Miyahara, Shinsuke; Almulki, Lama; Noda, Kousuke; Nakazawa, Toru; Hisatomi, Toshio; Nakao, Shintaro; Thomas, Kennard L.; Schering, Alexander; Zandi, Souska; Frimmel, Sonja; Tayyari, Faryan; Garland, Rebecca C.; Miller, Joan W.; Gragoudas, Evangelos S.; Masli, Sharmila; Hafezi-Moghadam, Ali.

In: FASEB Journal, Vol. 22, No. 6, 01.06.2008, p. 1973-1980.

Research output: Contribution to journalArticle

Miyahara, S, Almulki, L, Noda, K, Nakazawa, T, Hisatomi, T, Nakao, S, Thomas, KL, Schering, A, Zandi, S, Frimmel, S, Tayyari, F, Garland, RC, Miller, JW, Gragoudas, ES, Masli, S & Hafezi-Moghadam, A 2008, 'In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis', FASEB Journal, vol. 22, no. 6, pp. 1973-1980. https://doi.org/10.1096/fj.07-096891
Miyahara, Shinsuke ; Almulki, Lama ; Noda, Kousuke ; Nakazawa, Toru ; Hisatomi, Toshio ; Nakao, Shintaro ; Thomas, Kennard L. ; Schering, Alexander ; Zandi, Souska ; Frimmel, Sonja ; Tayyari, Faryan ; Garland, Rebecca C. ; Miller, Joan W. ; Gragoudas, Evangelos S. ; Masli, Sharmila ; Hafezi-Moghadam, Ali. / In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis. In: FASEB Journal. 2008 ; Vol. 22, No. 6. pp. 1973-1980.
@article{3d1a27fa2fe84788abbb105bb1b64753,
title = "In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis",
abstract = "Early detection of ocular inflammation may prevent the occurrence of structural damage or vision loss. Here, we introduce a novel noninvasive technique for molecular imaging and quantitative evaluation of endothelial injury in the choriocapillaris of live animals, which detects disease earlier than currently possible. Using an established model of ocular inflammation, endotoxin-induced uveitis (EIU), we visualized the rolling and adhesive interaction of fluorescent microspheres conjugated to recombinant P-selectin glycoprotein ligand-Ig (rPSGL-Ig) in choriocapillaris using a scanning laser ophthalmoscope (SLO). The number of rolling microspheres in the choriocapillaris peaked 4-10 h after LPS injection. The number of the accumulated microspheres peaked 4 h after LPS injection in the temporal choriocapillaris and 4 and 36 h after LPS injection in the central areas around the optic disk. Furthermore, we semiquantified the levels of P-selectin mRNA expression in the choroidal vessels by reverse transcription-PCR and found its pattern to match the functional microsphere interactions, with a peak at 4 h after LPS injection. These results indicate that PSGL-1-conjugated fluorescent microspheres allow specific detection of endothelial P-selectin expression in vivo and noninvasive assessment of endothelial injury. This technique may help to diagnose subclinical signs of ocular inflammatory diseases.",
author = "Shinsuke Miyahara and Lama Almulki and Kousuke Noda and Toru Nakazawa and Toshio Hisatomi and Shintaro Nakao and Thomas, {Kennard L.} and Alexander Schering and Souska Zandi and Sonja Frimmel and Faryan Tayyari and Garland, {Rebecca C.} and Miller, {Joan W.} and Gragoudas, {Evangelos S.} and Sharmila Masli and Ali Hafezi-Moghadam",
year = "2008",
month = "6",
day = "1",
doi = "10.1096/fj.07-096891",
language = "English",
volume = "22",
pages = "1973--1980",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis

AU - Miyahara, Shinsuke

AU - Almulki, Lama

AU - Noda, Kousuke

AU - Nakazawa, Toru

AU - Hisatomi, Toshio

AU - Nakao, Shintaro

AU - Thomas, Kennard L.

AU - Schering, Alexander

AU - Zandi, Souska

AU - Frimmel, Sonja

AU - Tayyari, Faryan

AU - Garland, Rebecca C.

AU - Miller, Joan W.

AU - Gragoudas, Evangelos S.

AU - Masli, Sharmila

AU - Hafezi-Moghadam, Ali

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Early detection of ocular inflammation may prevent the occurrence of structural damage or vision loss. Here, we introduce a novel noninvasive technique for molecular imaging and quantitative evaluation of endothelial injury in the choriocapillaris of live animals, which detects disease earlier than currently possible. Using an established model of ocular inflammation, endotoxin-induced uveitis (EIU), we visualized the rolling and adhesive interaction of fluorescent microspheres conjugated to recombinant P-selectin glycoprotein ligand-Ig (rPSGL-Ig) in choriocapillaris using a scanning laser ophthalmoscope (SLO). The number of rolling microspheres in the choriocapillaris peaked 4-10 h after LPS injection. The number of the accumulated microspheres peaked 4 h after LPS injection in the temporal choriocapillaris and 4 and 36 h after LPS injection in the central areas around the optic disk. Furthermore, we semiquantified the levels of P-selectin mRNA expression in the choroidal vessels by reverse transcription-PCR and found its pattern to match the functional microsphere interactions, with a peak at 4 h after LPS injection. These results indicate that PSGL-1-conjugated fluorescent microspheres allow specific detection of endothelial P-selectin expression in vivo and noninvasive assessment of endothelial injury. This technique may help to diagnose subclinical signs of ocular inflammatory diseases.

AB - Early detection of ocular inflammation may prevent the occurrence of structural damage or vision loss. Here, we introduce a novel noninvasive technique for molecular imaging and quantitative evaluation of endothelial injury in the choriocapillaris of live animals, which detects disease earlier than currently possible. Using an established model of ocular inflammation, endotoxin-induced uveitis (EIU), we visualized the rolling and adhesive interaction of fluorescent microspheres conjugated to recombinant P-selectin glycoprotein ligand-Ig (rPSGL-Ig) in choriocapillaris using a scanning laser ophthalmoscope (SLO). The number of rolling microspheres in the choriocapillaris peaked 4-10 h after LPS injection. The number of the accumulated microspheres peaked 4 h after LPS injection in the temporal choriocapillaris and 4 and 36 h after LPS injection in the central areas around the optic disk. Furthermore, we semiquantified the levels of P-selectin mRNA expression in the choroidal vessels by reverse transcription-PCR and found its pattern to match the functional microsphere interactions, with a peak at 4 h after LPS injection. These results indicate that PSGL-1-conjugated fluorescent microspheres allow specific detection of endothelial P-selectin expression in vivo and noninvasive assessment of endothelial injury. This technique may help to diagnose subclinical signs of ocular inflammatory diseases.

UR - http://www.scopus.com/inward/record.url?scp=44949232186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949232186&partnerID=8YFLogxK

U2 - 10.1096/fj.07-096891

DO - 10.1096/fj.07-096891

M3 - Article

C2 - 18216288

AN - SCOPUS:44949232186

VL - 22

SP - 1973

EP - 1980

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -