In vivo imaging of mitochondrial function in methamphetamine-treated rats

Takeshi Shiba, Mayumi Yamato, Wataru Kudo, Toshiaki Watanabe, Hideo Utsumi, Ken-Ichi Yamada

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Abuse of the powerfully addictive psychostimulant, methamphetamine, occurs worldwide. Recent studies have suggested that methamphetamine-induced dopaminergic neurotoxicity is related to oxidative stress. In response to nerve activation, the mitochondrial respiratory chain is rapidly activated. The enhancement of mitochondrial respiratory chain activation may induce oxidative stress in the brain. However, there is little experimental evidence regarding the mitochondrial function after methamphetamine administration in vivo. Here, we evaluated whether a single administration of methamphetamine induces ATP consumption and overactivation of mitochondria. We measured mitochondrial function in two different ways: by monitoring oxygen partial pressure using an oxygen-selective electrode, and by imaging of redox reactions using a nitroxyl radical (i.e., nitroxide) coupled with Overhauser-enhanced magnetic resonance imaging (OMRI). A single administration of methamphetamine to Wistar rats induced dopaminergic nerve activation, ATP consumption and an increase in mitochondrial respiratory chain function in both the striatum and cortex. Furthermore, antioxidant TEMPOL prevented the increase in mitochondrial oxidative damage and methamphetamine-induced sensitization. These findings suggest that energy-supplying reactions after dopaminergic nerve activation are associated with oxidative stress in both the striatum and cortex, leading to abnormal behavior.

Original languageEnglish
Pages (from-to)866-872
Number of pages7
JournalNeuroImage
Volume57
Issue number3
DOIs
Publication statusPublished - Aug 1 2011

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Methamphetamine
Electron Transport
Oxidative Stress
Adenosine Triphosphate
Oxygen
Partial Pressure
Oxidation-Reduction
Wistar Rats
Mitochondria
Electrodes
Antioxidants
Magnetic Resonance Imaging
Brain

All Science Journal Classification (ASJC) codes

  • Neurology
  • Cognitive Neuroscience

Cite this

In vivo imaging of mitochondrial function in methamphetamine-treated rats. / Shiba, Takeshi; Yamato, Mayumi; Kudo, Wataru; Watanabe, Toshiaki; Utsumi, Hideo; Yamada, Ken-Ichi.

In: NeuroImage, Vol. 57, No. 3, 01.08.2011, p. 866-872.

Research output: Contribution to journalArticle

Shiba, Takeshi ; Yamato, Mayumi ; Kudo, Wataru ; Watanabe, Toshiaki ; Utsumi, Hideo ; Yamada, Ken-Ichi. / In vivo imaging of mitochondrial function in methamphetamine-treated rats. In: NeuroImage. 2011 ; Vol. 57, No. 3. pp. 866-872.
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