TY - JOUR
T1 - In vivo imaging of oxidative stress in the kidney of diabetic mice and its normalization by angiotensin II type 1 receptor blocker
AU - Sonta, Toshiyo
AU - Inoguchi, Toyoshi
AU - Matsumoto, Shingo
AU - Yasukawa, Keiji
AU - Inuo, Mieko
AU - Tsubouchi, Hirotaka
AU - Sonoda, Noriyuki
AU - Kobayashi, Kunihisa
AU - Utsumi, Hideo
AU - Nawata, Hajime
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (No.16590888) from the Ministry of Education, Science and Culture, Japan.
PY - 2005/5/6
Y1 - 2005/5/6
N2 - This study was undertaken to evaluate oxidative stress in the kidney of diabetic mice by electron spin resonance (ESR) imaging technique. Oxidative stress in the kidney was evaluated as organ-specific reducing activity with the signal decay rates of carbamoyl-PROXYL probe using ESR imaging. The signal decay rates were significantly faster in corresponding image pixels of the kidneys of streptozotocin-induced diabetic mice than in those of controls. This technique further demonstrated that administration of angiotensin II type 1 receptor blocker (ARB), olmesartan (5 mg/kg), completely restored the signal decay rates in the diabetic kidneys to control values. In conclusion, this study provided for the first time the in vivo evidence for increased oxidative stress in the kidneys of diabetic mice and its normalization by ARB as evaluated by ESR imaging. This technique would be useful as a means of further elucidating the role of oxidative stress in diabetic nephropathy.
AB - This study was undertaken to evaluate oxidative stress in the kidney of diabetic mice by electron spin resonance (ESR) imaging technique. Oxidative stress in the kidney was evaluated as organ-specific reducing activity with the signal decay rates of carbamoyl-PROXYL probe using ESR imaging. The signal decay rates were significantly faster in corresponding image pixels of the kidneys of streptozotocin-induced diabetic mice than in those of controls. This technique further demonstrated that administration of angiotensin II type 1 receptor blocker (ARB), olmesartan (5 mg/kg), completely restored the signal decay rates in the diabetic kidneys to control values. In conclusion, this study provided for the first time the in vivo evidence for increased oxidative stress in the kidneys of diabetic mice and its normalization by ARB as evaluated by ESR imaging. This technique would be useful as a means of further elucidating the role of oxidative stress in diabetic nephropathy.
UR - http://www.scopus.com/inward/record.url?scp=20144376707&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20144376707&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.02.174
DO - 10.1016/j.bbrc.2005.02.174
M3 - Article
C2 - 15796899
AN - SCOPUS:20144376707
VL - 330
SP - 415
EP - 422
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -
