In vivo interaction of human MCM heterohexameric complexes with chromatin: Possible involvement of ATP

Masatoshi Fujita, Tohru Kiyono, Yasuyuki Hayashi, Masahide Ishibashi

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The MCM protein family, which consists of at least six members, has been implicated in the regulatory machinery causing DNA to replicate once in the S phase. Mammalian MCM proteins are present in the nucleus in two different forms, one extractable by nonionic detergents and the other resistant to such extraction. The latter is assumed to be tightly associated with nuclear structures and released at the time of initiation of replication. However, details of the mode of binding remain unclear. In the present study, we found that, in nonionic detergent-permeabilized nuclei, the association of human MCM (hMCM) proteins with them could be stabilized by the addition of ATP. The hMCMs bound to the nuclei in the presence of ATP were released by digestion with nucleases, suggesting that they are chromatin-associated. The nuclease- directed solubilization of the chromatin-bound hMCMs thus provided a means to analyze them as well as soluble hMCMs by co-immunoprecipitation. The results indicate that the six hMCM members exist as heterocomplexes, whether bound or unbound. We therefore propose that hMCM proteins may function in DNA replication as heterohexamers associated with chromatin and that ATP is possibly involved in the association. Nuclease digestion-immunoprecipitation techniques of the type described here should facilitate further elucidation of the mode of interaction between hMCMs and chromatin.

Original languageEnglish
Pages (from-to)10928-10935
Number of pages8
JournalJournal of Biological Chemistry
Volume272
Issue number16
DOIs
Publication statusPublished - Apr 18 1997
Externally publishedYes

Fingerprint

Multicarrier modulation
Chromatin
Adenosine Triphosphate
Immunoprecipitation
Detergents
Digestion
Proteins
DNA Replication
S Phase
DNA
Machinery

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

In vivo interaction of human MCM heterohexameric complexes with chromatin : Possible involvement of ATP. / Fujita, Masatoshi; Kiyono, Tohru; Hayashi, Yasuyuki; Ishibashi, Masahide.

In: Journal of Biological Chemistry, Vol. 272, No. 16, 18.04.1997, p. 10928-10935.

Research output: Contribution to journalArticle

Fujita, Masatoshi ; Kiyono, Tohru ; Hayashi, Yasuyuki ; Ishibashi, Masahide. / In vivo interaction of human MCM heterohexameric complexes with chromatin : Possible involvement of ATP. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 16. pp. 10928-10935.
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