In vivo magnetic resonance imaging of atherosclerotic lesions with a newly developed evans blue-DTPA-gadolinium contrast medium in apolipoprotein-E- deficient mice

Satoshi Yasuda, Kenjiro Ikuta, Toyokazu Uwatoku, Keiji Oi, Kohtaro Abe, Fuminori Hyodo, Kengo Yoshimitsu, Kohtaro Sugimura, Hideo Utsumi, Yoshiki Katayama, Hiroaki Shimokawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Magnetic resonance imaging (MRI) contrast agents that specifically detect atherosclerotic plaque may be useful for the noninvasive detection of the plaque. We have recently developed a new contrast agent, Evans blue-DTPA-gadolinium (EB-DTPA-Gd), which selectively accumulates vascular lesions with endothelial removal. In this study, we examined whether EB-DTPA-Gd is also useful for in vivo imaging of atherosclerotic plaques. Methods: We used male apolipoprotein-E-deficient (ApoE-/-) mice of different ages (3, 6 and 12 months old) and age-matched male wild-type mice. After a single intravenous administration of EB-DTPA-Gd (160 μM/kg body weight), MRI T1 signal was obtained in vivo. Results: Increased signal intensity in the aortic wall was noted within 10-20 min after intravenous injection of EB-DTPA-Gd and was maintained for 30 min. The MRI enhancement in the aorta of ApoE-/- mice was increased in accordance with age, whereas no such enhancement was noted in wild-type mice. Histological examination demonstrated that there was a topological correlation between the site of MRI enhancement and that of atherosclerotic plaque. Conclusions: These results indicate that EB-DTPA-Gd is a useful MRI contrast medium for the in vivo detection of atherosclerotic plaques.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalJournal of Vascular Research
Volume45
Issue number2
DOIs
Publication statusPublished - Feb 2008

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

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