TY - JOUR
T1 - In vivo optical access to olfactory sensory neurons in the mouse olfactory epithelium
AU - Inagaki, Shigenori
AU - Iwata, Ryo
AU - Imai, Takeshi
N1 - Funding Information:
This work was supported by grants from the PRESTO and CREST programs of the Japan Science and Technology Agency (JST), Japan (T.I.), the JSPS KAKENHI, Japan (grant numbers JP23680038, JP15H05572, JP15K14336, JP16K14568, JP16H06456, JP17H06261, and JP21H00205 to T.I., JP15K18353 to R.I., and JP21H02140 to S.I.), the Mochida Memorial Foundation for Medical and Pharmaceutical Research, intramural grant from RIKEN Center for Developmental Biology (T.I.), and Grant-in-Aid for JSPS Research Fellow, Japan (JP15J08987 to R.I. and JP18J00899 to S.I.). We thank M.N. Leiwe for comments on the manuscript. This protocol has been used in our research (Iwata et al., 2017; Inagaki et al., 2020).
Publisher Copyright:
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.
PY - 2021/6/20
Y1 - 2021/6/20
N2 - In neuroscience, it is fundamental to understand how sensory stimuli are translated into neural activity at the entry point of sensory systems. In the olfactory system, odorants inhaled into the nasal cavity are detected by ~1,000 types of odorant receptors (ORs) that are expressed by olfactory sensory neurons (OSNs). Since each OSN expresses only one type of odorant receptor, the odor-evoked responses reflect the interaction between odorants and the expressed OR. The responses of OSN somata are often measured by calcium imaging and electrophysiological techniques; however, previous techniques require tissue dissection or cell dissociation, rendering it difficult to investigate physiological responses. Here, we describe a protocol that allows us to observe odor-evoked responses of individual OSN somata in the mouse olfactory epithelium in vivo. Two-photon excitation through the thinned skull enables highly-sensitive calcium imaging using a genetically encoded calcium indicator, GCaMP. Recording of odor-evoked responses in OSN somata in freely breathing mice will be fundamental to understanding how odor information is processed at the periphery and higher circuits in the brain.
AB - In neuroscience, it is fundamental to understand how sensory stimuli are translated into neural activity at the entry point of sensory systems. In the olfactory system, odorants inhaled into the nasal cavity are detected by ~1,000 types of odorant receptors (ORs) that are expressed by olfactory sensory neurons (OSNs). Since each OSN expresses only one type of odorant receptor, the odor-evoked responses reflect the interaction between odorants and the expressed OR. The responses of OSN somata are often measured by calcium imaging and electrophysiological techniques; however, previous techniques require tissue dissection or cell dissociation, rendering it difficult to investigate physiological responses. Here, we describe a protocol that allows us to observe odor-evoked responses of individual OSN somata in the mouse olfactory epithelium in vivo. Two-photon excitation through the thinned skull enables highly-sensitive calcium imaging using a genetically encoded calcium indicator, GCaMP. Recording of odor-evoked responses in OSN somata in freely breathing mice will be fundamental to understanding how odor information is processed at the periphery and higher circuits in the brain.
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U2 - 10.21769/BioProtoc.4055
DO - 10.21769/BioProtoc.4055
M3 - Article
AN - SCOPUS:85113171481
VL - 11
JO - Bio-protocol
JF - Bio-protocol
SN - 2331-8325
IS - 12
M1 - e4055
ER -