In vivo patch-clamp analysis of sensory neuronal circuit in the rat superficial spinal dorsal horn

Hidemasa Furue, Go Kato, Ji Hoon Kim, Byung Il Min, Toshihiko Katafuchi, Megumu Yoshimura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In vivo whole-cell voltage-clamp recordings were made from substantia gelatinosa (SG, lamina II of the spinal cord) neurons to analyze modality-dependent excitatory and inhibitory synaptic responses and their receptive fields. All SG neurons examined exhibited spontaneous excitatory postsynaptic currents (EPSCs) at a holding potential of −70 mV. Pinch and touch stimuli applied to the skin of the hind limb elicited a barrage of EPSCs. The spontaneous and evoked EPSCs were blocked by superfusing CNQX, a non-NMDA receptor antagonist. At a holding potential of 0 mV, all SG neurons exhibited spontaneous inhibitory postsynaptic currents (IPSCs) which were inhibited by either bicuculline, a GABAA receptor-antagonist, or strychnine, a glycine-receptor antagonist. In most neurons, touch stimuli produced a barrage of IPSCs. These evoked IPSCs were inhibited either by superfusing strychnine or bicuculline. When EPSCs and IPSCs were recorded from the same neurons, the size of receptive fields of evoked IPSCs was larger than that of evoked EPSCs. These results suggest that innocuous mechanical stimulation applied to the skin adjacent to a painful point inhibits mechanical noxious transmission in the SG.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalInternational Congress Series
Volume1269
Issue numberC
DOIs
Publication statusPublished - Aug 1 2004

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Inhibitory Postsynaptic Potentials
Excitatory Postsynaptic Potentials
Neurons
Substantia Gelatinosa
Strychnine
Bicuculline
Touch
6-Cyano-7-nitroquinoxaline-2,3-dione
Glycine Receptors
GABA-A Receptor Antagonists
Skin
Spinal Cord
Extremities
Spinal Cord Dorsal Horn

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

In vivo patch-clamp analysis of sensory neuronal circuit in the rat superficial spinal dorsal horn. / Furue, Hidemasa; Kato, Go; Kim, Ji Hoon; Min, Byung Il; Katafuchi, Toshihiko; Yoshimura, Megumu.

In: International Congress Series, Vol. 1269, No. C, 01.08.2004, p. 69-72.

Research output: Contribution to journalArticle

Furue, Hidemasa ; Kato, Go ; Kim, Ji Hoon ; Min, Byung Il ; Katafuchi, Toshihiko ; Yoshimura, Megumu. / In vivo patch-clamp analysis of sensory neuronal circuit in the rat superficial spinal dorsal horn. In: International Congress Series. 2004 ; Vol. 1269, No. C. pp. 69-72.
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