TY - JOUR
T1 - In vivo redox metabolic imaging of mitochondria assesses disease progression in non-alcoholic steatohepatitis
AU - Nakata, Ryosuke
AU - Hyodo, Fuminori
AU - Murata, Masaharu
AU - Eto, Hinako
AU - Nakaji, Tomoko
AU - Kawano, Takahito
AU - Narahara, Sayoko
AU - Yasukawa, Keiji
AU - Akahoshi, Tomohiko
AU - Tomikawa, Morimasa
AU - Hashizume, Makoto
N1 - Funding Information:
This work was supported by the Medical Research and Development Programs Focused on Technology Transfer, Development of Advanced Measurement and Analysis Systems (SENTAN) from Japan Agency for Medical Research and Development, AMED Grant Number 162128; Health Labour Sciences Research Grant (Research on Publicly Essential Drugs and Medical Devices) from the Ministry of Health, Labour and Welfare of Japan; Special Coordination Funds for Promoting Science and Technology (SCF funding program “Innovation Center for Medical Redox Navigation”); Grant-in-Aid for Scientific Research, Grant Numbers 24300172, 16H05079 and 16K16409, from the Ministry of Education, Culture, Sports, Science and Technology of Japan; and JSPS Grant-in-Aid for Scientific Research on Innovative Areas (Multidisciplinary Computational Anatomy) JSPS KAKENHI, Grant Number 26108010.
Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Given the rising incidence of non-alcoholic fatty liver disease (NAFLD) in both adults and children, the development of a non-invasive diagnostic method for assessing disease progression to non-alcoholic steatohepatitis (NASH) has become an important research goal. Currently available non-invasive imaging technologies are only able to assess fat accumulation in the liver. Therefore, these methods are not suitable for a precise diagnosis of NASH. The standard diagnostic technique for NASH, liver biopsy, has several drawbacks, including the higher risk of complications that accompanies invasive procedures. Here, we demonstrated that in vivo mitochondrial redox metabolism was dramatically altered at an early stage, before histopathological changes, and NASH could be accurately diagnosed by in vivo dynamic nuclear polarization-magnetic resonance imaging, with carbamoyl-PROXYL as a molecular imaging probe. In addition, this technique was feasible for the diagnosis of NASH compared with histopathological findings from biopsies. Our data reveal a novel method for monitoring the dynamics of redox metabolic changes in NAFLD/NASH.
AB - Given the rising incidence of non-alcoholic fatty liver disease (NAFLD) in both adults and children, the development of a non-invasive diagnostic method for assessing disease progression to non-alcoholic steatohepatitis (NASH) has become an important research goal. Currently available non-invasive imaging technologies are only able to assess fat accumulation in the liver. Therefore, these methods are not suitable for a precise diagnosis of NASH. The standard diagnostic technique for NASH, liver biopsy, has several drawbacks, including the higher risk of complications that accompanies invasive procedures. Here, we demonstrated that in vivo mitochondrial redox metabolism was dramatically altered at an early stage, before histopathological changes, and NASH could be accurately diagnosed by in vivo dynamic nuclear polarization-magnetic resonance imaging, with carbamoyl-PROXYL as a molecular imaging probe. In addition, this technique was feasible for the diagnosis of NASH compared with histopathological findings from biopsies. Our data reveal a novel method for monitoring the dynamics of redox metabolic changes in NAFLD/NASH.
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U2 - 10.1038/s41598-017-17447-2
DO - 10.1038/s41598-017-17447-2
M3 - Article
C2 - 29215054
AN - SCOPUS:85038081920
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 17170
ER -