In vivo spectral-spatial imaging for oxygen mapping using single-point, time-domain electron paramagnetic resonance

Sankaran Subramanian, Calvin A. Johnson, Nallathamby Devasahayam, Ken Ichiro Matsumoto, Fuminori Hyodo, John Cook, Murali C. Krishna

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)

    Abstract

    Electron Paramagnetic Resonance (EPR) is a spectroscopic technique that detects and characterizes molecules with unpaired electrons (i.e., free radicals). Although it is closely related to nuclear magnetic resonance (NMR) spectroscopy, EPR is still under development as an imaging modality. Unlike other imaging modalities, EPR is able to take direct measurements of tissue oxygen concentration in a manner that is not dependent on complex biological processes such as ligand binding specificity or tracer metabolism. We describe the use of single-point imaging (SPI) in radiofrequency, Fourier-transform EPR. We present the techniques for performing oxygen imaging using EPR with phantoms and in vivo. We present results from a study in which the two mouse legs (one normal and the other with tumor) were imaged over time as the mouse breathed alternately air, carbogen (95% O 2, 5% CO 2), and then air again. The reconstructed images demonstrate that the SPI EPR imaging technique readily distinguishes between the normal and tumor legs and can track the changes in tissue oxygen concentration in response to percentage of oxygen in breathing gas.

    Original languageEnglish
    Title of host publication2006 3rd IEEE International Symposium on Biomedical Imaging
    Subtitle of host publicationFrom Nano to Macro - Proceedings
    Pages1096-1099
    Number of pages4
    Publication statusPublished - Nov 17 2006
    Event2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Arlington, VA, United States
    Duration: Apr 6 2006Apr 9 2006

    Publication series

    Name2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings
    Volume2006

    Other

    Other2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro
    CountryUnited States
    CityArlington, VA
    Period4/6/064/9/06

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    All Science Journal Classification (ASJC) codes

    • Engineering(all)

    Cite this

    Subramanian, S., Johnson, C. A., Devasahayam, N., Matsumoto, K. I., Hyodo, F., Cook, J., & Krishna, M. C. (2006). In vivo spectral-spatial imaging for oxygen mapping using single-point, time-domain electron paramagnetic resonance. In 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings (pp. 1096-1099). [1625113] (2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings; Vol. 2006).