Inactivation of bacillus subtilis spores by carbonation with glycerin fatty acid esters

Rina Hirokado, Seiji Noma, Nobuaki Soh, Noriyuki Igura, Mitsuya Shimoda, Nobuyuki Hayashi

Research output: Contribution to journalArticle

Abstract

We investigated the effect of carbonation treatment with heating (CH) in the presence of glycerin fatty acid esters on Bacillus subtilis spores. The inactivation effect of CH was the highest in the presence of monoglycerol monocaprate (MC10). Monoglycerol monolaurate showed the highest bacteriostatic effect. The resistance of spores to CH with MC10 did not change with pre-heat activation. Freshly prepared spores from the surviving spores following CH with MC10 showed resistance similar to that of normal spores. The resistance of spores might be affected by the composition of the spore-forming medium. CH with MC10 enhanced adhesion of MC10 to spores and dipicolinic acid (DPA) release from spores, but did not induce DNA fragmentation. Inactivation of B. Subtilis spores by CH with MC10 may be attributable to the impairment of physiological germination and/or decrease in heat resistance, which were induced by enhanced MC10 adhesion and the resultant DPA release.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalFood Science and Technology Research
Volume24
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

carbonation
Carbonation
fatty acid esters
Bacilli
Bacillus subtilis
Spores
Glycerol
Fatty acids
glycerol
Esters
inactivation
Fatty Acids
Adhesion
spores
Acids
Heat resistance
DNA
Chemical activation
Heating
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Cite this

Inactivation of bacillus subtilis spores by carbonation with glycerin fatty acid esters. / Hirokado, Rina; Noma, Seiji; Soh, Nobuaki; Igura, Noriyuki; Shimoda, Mitsuya; Hayashi, Nobuyuki.

In: Food Science and Technology Research, Vol. 24, No. 3, 01.01.2018, p. 455-463.

Research output: Contribution to journalArticle

Hirokado, Rina ; Noma, Seiji ; Soh, Nobuaki ; Igura, Noriyuki ; Shimoda, Mitsuya ; Hayashi, Nobuyuki. / Inactivation of bacillus subtilis spores by carbonation with glycerin fatty acid esters. In: Food Science and Technology Research. 2018 ; Vol. 24, No. 3. pp. 455-463.
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